Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells

doi:10.1039/d0an00071j

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells
	Li, Jia ; Wang, Yuhui ; Sun, Shan ; Lv, A-Man ; Jiang, Kai ; Li, Yike ; Li, Zhongjun ; Lin, Hengwei
刊名	ANALYST
	2020
卷号	145 期号:8 页码:2982-2987
关键词	RESONANCE ENERGY-TRANSFER CANCER-THERAPY QUANTUM DOTS GLUTATHIONE DESIGN REDOX NANOCLUSTERS CLEAVAGE PROBES SENSOR
DOI	10.1039/d0an00071j
英文摘要	Herein, we report a turn-on fluorimetric nanoprobe for intracellular glutathione (GSH) imaging. The principle of this probe is designed on the basis of the selective reduction between GSH and disulfide bond-based self-crosslinked red emissive carbon dots (abbreviated as SCCDs). The nanoprobe (i.e., SCCDs) was facilely fabricated from thiol-modified carbon dots (CDs) through oxidation in the presence of H2O2, and its fluorescence was greatly reduced due to the effect of aggregation induced quenching (AIQ). However, in the presence of GSH, the SCCDs were separated into many single CDs. As a result, the fluorescence of the nanoprobe was recovered in a GSH concentration-dependent manner, which is the basis for the quantitative analysis of GSH. The nanoprobe shows excellent specificity and a linear range from 0 to 0.15 mM towards GSH with a limit of detection (LOD) of 5.7 mu M. Finally, the nanoprobe was demonstrated to have extremely low cytotoxicity, and was successfully applied for monitoring the GSH level in living cells. This work would provide a promising probe for the research of GSH in cytobiology.
学科主题	Chemistry
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/19779]
专题	2020专题
作者单位	1.Lin, HW (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Ningbo 315201, Peoples R China. 2.Li, YK (corresponding author), Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China. 3.Wang, YH
推荐引用方式 GB/T 7714	Li, Jia,Wang, Yuhui,Sun, Shan,et al. Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells[J]. ANALYST,2020,145(8):2982-2987.
APA	Li, Jia.,Wang, Yuhui.,Sun, Shan.,Lv, A-Man.,Jiang, Kai.,...&Lin, Hengwei.(2020).Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells.ANALYST,145(8),2982-2987.
MLA	Li, Jia,et al."Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells".ANALYST 145.8(2020):2982-2987.