N-glycopeptide Signatures of IgA(2) in Serum from Patients with Hepatitis B Virus-related Liver Diseases

doi:10.1074/mcp.RA119.001722

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	N-glycopeptide Signatures of IgA(2) in Serum from Patients with Hepatitis B Virus-related Liver Diseases
	Zhang, Shu 1,2; Cao, Xinyi 3; Liu, Chao 6; Li, Wei 3; Zeng, Wenfeng 7; Li, Baiwen 8; Chi, Hao 7; Liu, Mingqi 3; Qin, Xue 9; Tang, Lingyi 10
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2019-11-01
卷号	18 期号:11 页码:2262-2272
ISSN号	1535-9476
DOI	10.1074/mcp.RA119.001722
英文摘要	N-glycosylation alteration has been reported in liver diseases. Characterizing N-glycopeptides that correspond to N-glycan structure with specific site information enables better understanding of the molecular pathogenesis of liver damage and cancer. Here, unbiased quantification of N-glycopeptides of a cluster of serum glycoproteins with 40-55 kDa molecular weight (40-kDa band) was investigated in hepatitis B virus (HBV)-related liver diseases. We used an N-glycopeptide method based on O-18/O-16 C-terminal labeling to obtain 82 comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC) and liver cirrhosis (LC). Then, multiple reaction monitoring (MRM) was performed to quantify N-glycopeptide relative to the protein content, especially in the healthy donor-HBV-LC-HCC cascade. TPLTAN(205)ITK (H5N5S1F1) and (H5N4S2F1) corresponding to the glycopeptides of IgA(2) were significantly elevated in serum from patients with HBV infection and even higher in HBV-related LC patients, as compared with healthy donor. In contrast, the two glycopeptides of IgA(2) fell back down in HBV-related HCC patients. In addition, the variation in the abundance of two glycopeptides was not caused by its protein concentration. The altered N-glycopeptides might be part of a unique glycan signature indicating an IgA-mediated mechanism and providing potential diagnostic clues in HBV-related liver diseases.
资助项目	National Key Research and Development Program of China[2016YFA0501303] ; National Science and Technology Major Project of China[2018ZX10302205-003] ; National Natural Science Foundation of China[21505022] ; National Natural Science Foundation of China[81522036] ; National Natural Science Foundation of China[31700727] ; Zhongshan Hospital[2017ZSGG08]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000494461000009
内容类型	期刊论文
源URL	[http://119.78.100.204/handle/2XEOYT63/14899]
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Gao, Qiang; Lu, Haojie
作者单位	1.Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Shanghai 200032, Peoples R China 2.Fudan Univ, Key Lab Carcinogenesis & Canc Invas, Minist Educ, Shanghai 200032, Peoples R China 3.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China 4.Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China 5.Fudan Univ, NHC Key Lab Glycoconjugates Res, Shanghai 200032, Peoples R China 6.Beihang Univ, Beijing Adv Innovat Ctr Precis Med, Beijing 100083, Peoples R China 7.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 8.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Gastroenterol, Shanghai 201620, Peoples R China 9.Guangxi Med Univ, Dept Clin Lab, Affiliated Hosp 1, Nanning 530021, Guangxi, Peoples R China 10.Univ Texas Hlth Sci Ctr Houston, Sch Biomed Informat, Houston, TX 77030 USA
推荐引用方式 GB/T 7714	Zhang, Shu,Cao, Xinyi,Liu, Chao,et al. N-glycopeptide Signatures of IgA(2) in Serum from Patients with Hepatitis B Virus-related Liver Diseases[J]. MOLECULAR & CELLULAR PROTEOMICS,2019,18(11):2262-2272.
APA	Zhang, Shu.,Cao, Xinyi.,Liu, Chao.,Li, Wei.,Zeng, Wenfeng.,...&Lu, Haojie.(2019).N-glycopeptide Signatures of IgA(2) in Serum from Patients with Hepatitis B Virus-related Liver Diseases.MOLECULAR & CELLULAR PROTEOMICS,18(11),2262-2272.
MLA	Zhang, Shu,et al."N-glycopeptide Signatures of IgA(2) in Serum from Patients with Hepatitis B Virus-related Liver Diseases".MOLECULAR & CELLULAR PROTEOMICS 18.11(2019):2262-2272.