The regeneration of Acer rubrumL. "October Glory" through embryonic callus

doi:10.1186/s12870-020-02496-z

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所

	The regeneration of Acer rubrumL. "October Glory" through embryonic callus
	Dai, Chong-wen 1; Yan, Yang-yang 2; Liu, Yu-min 1; Liu, Ya-min 1; Deng, Yuan-wei 1; Yao, Hong-yu 1
刊名	BMC PLANT BIOLOGY
	2020-07-02
卷号	20 期号:1 页码:309
关键词	Acer rubrumL "October Glory " Tissue culture Embryogenic callus Regeneration
ISSN号	1471-2229
DOI	10.1186/s12870-020-02496-z
产权排序	2
文献子类	Article
英文摘要	Background Tissue culture and rapid propagation technology is an important way to solve the difficulties of plant propagation. This experiment aims to explore the appropriate conditions at each stage of the red maple's tissue culture process and to obtain plantlets, thus providing a theoretical basis for the establishment of the red maple's tissue culture system. Results The results showed that the stem segment is the most suitable explant for inducing embryogenic callus. The MS (Murashige&Skoog) + 0.8 mg/L TDZ (Thidiazuron) + 1.0 mg/L 6-BA (6-Benzylaminopurine) + 0.5 mg/L IAA(Indole-3-acetic acid) + 35 g/L sucrose+ 7.5 g/L semi-fixed medium was the best for callus formation. When selecting type VI callus as embryonic callus induction material, MS + 0.6 mg/L TDZ + 0.5 mg/L 6-BA + 2.0 mg/L IAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS + 1.0 mg/L TDZ + 3.0 mg/L 6-BA+ 0.2 mg/L NAA (1-Naphthaleneacetic acid) + 1.2 mg/L IAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium. The induction rate of adventitious roots in MS + 0.6 mg/L TDZ + 1.0 mg/L 6-BA+ 3 mg/L NAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium was the highest, reaching 76%. Conclusions In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be sub-cultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.
资助项目	Key Project of Forestry Science and Technology of Chongqing Municipality[cfa2018lykj05] ; Fundamental Research Funds for the Central Universities[SWU119066] ; Application Development Plan of Chongqing Municipality[cstc2014yykfB80015]
WOS关键词	SOMATIC EMBRYOGENESIS ; PLANT ; MATURATION ; CULTURE ; GERMINATION ; CYTOKININ ; CELLS ; AUXIN
WOS研究方向	Plant Sciences
语种	英语
出版者	BMC
WOS记录号	WOS:000551892000001
资助机构	Key Project of Forestry Science and Technology of Chongqing Municipality ; Fundamental Research Funds for the Central Universities ; Application Development Plan of Chongqing Municipality
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/35278]
专题	中国科学院水利部成都山地灾害与环境研究所
通讯作者	Liu, Yu-min
作者单位	1.Southwest Univ, Resources & Environm Dept, Three Gorges Reservoir Area Lab Ecol Environm, Minist Educ, Chongqing 400715, Peoples R China; 2.Inst Mt Hazards & Environm, Chengdu, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Dai, Chong-wen,Yan, Yang-yang,Liu, Yu-min,et al. The regeneration of Acer rubrumL. "October Glory" through embryonic callus[J]. BMC PLANT BIOLOGY,2020,20(1):309.
APA	Dai, Chong-wen,Yan, Yang-yang,Liu, Yu-min,Liu, Ya-min,Deng, Yuan-wei,&Yao, Hong-yu.(2020).The regeneration of Acer rubrumL. "October Glory" through embryonic callus.BMC PLANT BIOLOGY,20(1),309.
MLA	Dai, Chong-wen,et al."The regeneration of Acer rubrumL. "October Glory" through embryonic callus".BMC PLANT BIOLOGY 20.1(2020):309.