Investigation into the content change and distribution of active components in Cordyceps sinensis with growth cycle by direct TOF-SIMS detection

doi:10.1016/j.microc.2021.106026

	Investigation into the content change and distribution of active components in Cordyceps sinensis with growth cycle by direct TOF-SIMS detection
	Xia, Meng-Chan 3; Zhan, Qin 4,5; Cai, Lesi 1; Wu, Jiang 5; Yang, Lu 1; Sun, Suqin 1; Liang, Handong 5; Li, Zhanping 1,2
刊名	MICROCHEMICAL JOURNAL
	2021-05-01
卷号	164 页码:5
关键词	TOF-SIMS Cordyceps sinensis Active components Content change Growth cycle
ISSN号	0026-265X
DOI	10.1016/j.microc.2021.106026
通讯作者	Cai, Lesi(cls15@mails.tsinghua.edu.cn) ; Liang, Handong(hdl6688@vip.sina.com) ; Li, Zhanping(zhanpingli@mail.tsinghua.edu.cn)
英文摘要	Analyzing the content of active components in Chinese medicines is crucial to evaluate their medicinal value and further promote the development of pharmaceutical technology. In this work, Cordyceps sinensis, a precious Chinese medicine, was characterized by time-of-flight secondary ion mass spectrometry (TOF-SIMS) to measure the content change of amino acids, nucleosides, mannitol and ergosterol with its growth cycle. Direct and sensitive detection of these active components in Cordyceps sinensis was realized by TOF-SIMS without tedious pretreatment process, such as repeated extraction and purification. Due to complex metabolic regulation, amino acids, nucleosides, mannitol and ergosterol showed different changing trends with growth cycle of Cordyceps sinensis. We found that the changing trend of the same active substance in different parts of Cordyceps sinensis varied dramatically. For instance, with the growth cycle of Cordyceps sinensis, the content of mannitol maintained steady growth in the stroma, while fell first and rose later in the worm body. In some growth stage, the content of mannitol in the worm body was about 10 times higher than that in the stroma. We provided a systematically analytical method and rich data base for further research of the extraction of active components and artificial cultivation of Cordyceps sinensis in this study. Meanwhile, TOF-SIMS is verified to be a sensitive, efficient and convenient method to characterize active components in traditional Chinese medicines.
资助项目	National Key R&D Program of China[2018YFA0702600]
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000635089400006
资助机构	National Key R&D Program of China
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/161836]
专题	中国科学院地理科学与资源研究所
通讯作者	Cai, Lesi; Liang, Handong; Li, Zhanping
作者单位	1.Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China 2.Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 4.Guzhen Cty 1 Middle Sch, Bengbu 233700, Anhui, Peoples R China 5.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Xia, Meng-Chan,Zhan, Qin,Cai, Lesi,et al. Investigation into the content change and distribution of active components in Cordyceps sinensis with growth cycle by direct TOF-SIMS detection[J]. MICROCHEMICAL JOURNAL,2021,164:5.
APA	Xia, Meng-Chan.,Zhan, Qin.,Cai, Lesi.,Wu, Jiang.,Yang, Lu.,...&Li, Zhanping.(2021).Investigation into the content change and distribution of active components in Cordyceps sinensis with growth cycle by direct TOF-SIMS detection.MICROCHEMICAL JOURNAL,164,5.
MLA	Xia, Meng-Chan,et al."Investigation into the content change and distribution of active components in Cordyceps sinensis with growth cycle by direct TOF-SIMS detection".MICROCHEMICAL JOURNAL 164(2021):5.