Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming

doi:10.1016/j.soilbio.2020.107720

	Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming
	Cui, Jun 1,2,10,11; Zhu, Zhenke 1,10,11; Xu, Xingliang 9; Liu, Shoulong 1,10; Jones, Davey L.8; Kuzyakov, Yakov 4,5,6,7; Shibistova, Olga 3; Wu, Jinshui 1,10; Ge, Tida 1,10,11
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2020-03-01
卷号	142 页码:13
关键词	Soil carbon Priming effects Glucose mineralization Compound-specific C-13-PLFA analysis Necromass recycling Stoichiometric imbalance
ISSN号	0038-0717
DOI	10.1016/j.soilbio.2020.107720
通讯作者	Ge, Tida(gtd@isa.ac.cn)
英文摘要	The impact of increasing amounts of labile C input on priming effects (PE) on soil organic matter (SOM) mineralization remains unclear, particularly under anoxic conditions and under high C input common in microbial hotspots. PE and their mechanisms were investigated by a 60-day incubation of three flooded paddy soils amended with(13)C-labeled glucose equivalent to 50-500% of microbial biomass C (MBC). PE (14-55% of unamended soil) peaked at moderate glucose addition rates (i.e., 50-300% of MBC). Glucose addition above 300% of MBC suppressed SOM mineralization but intensified microbial N acquisition, which contradicted the common PE mechanism of accelerating SOM decomposition for N-supply (frequently termed as "N mining"). Particularly at glucose input rate higher than 3 g kg(-1) (i.e., 300-500% of MBC), mineral N content dropped on day 2 close to zero (1.1-2.5 mg N kg(-1)) because of microbial N immobilization. To cope with the N limitation, microorganisms greatly increased N-acetyl glucosaminidase and leucine aminopeptidase activities, while SOM decomposition decreased. Several discrete peaks of glucose-derived CO2 (contributing >80% to total CO2) were observed between days 13-30 under high glucose input (300-500% of MBC), concurrently with CH4 peaks. Such CO2 dynamics was distinct from the common exponential decay pattern, implicating the recycling and mineralization of C-13-enriched microbial necromass driven by glucose addition. Therefore, N recycling from necromass was hypothesized as a major mechanism to alleviate microbial N deficiency without SOM priming under excess labile C input. Compound-specific C-13-PLFA confirmed the redistribution of glucose-derived C among microbial groups, i.e., necromass recycling. Following glucose input, more than 4/5 of total C-13-PLFA was in the gram-negative and some non-specific bacteria, suggesting these microorganisms as r-strategists capable of rapidly utilizing the most labile C. However, their C-13-PLFA content decreased by 70% after 60 days, probably as a result of death of these r-strategists. On the contrary, the C-13-PLFA in gram-positive bacteria, actinomycetes and fungi (K-strategists) was initially minimal but increased by 0.5-5 folds between days 2 and 60. Consequently, the necromass of dead r-strategists provided a high-quality C-N source to the K-strategists. We conclude that under severe C excess, N recycling from necromass is a much more efficient microbial strategy to cover the acute N demand than N acquisition from the recalcitrant SOM.
资助项目	National Key Research and Development Program of China[2017YFD0800104] ; National Natural Science Foundation of China[41430860] ; National Natural Science Foundation of China[41771337] ; National Natural Science Foundation of China[41977093] ; National Natural Science Foundation of China[31872695] ; State Key Laboratory of Organic Geochemistry, GIGCAS[SKLOG-201728] ; Hunan Province Base for Scientific and Technological Innovation Cooperation[2018WK4012] ; Youth Innovation Team Project of Institute of Subtropical Agriculture, Chinese Academy of Sciences[2017QNCXTD_GTD] ; NSFC-RFBR joint project[N 19-54-53026] ; Innovation Groups of National Natural Science Foundation of Hunan Province[2019JJ10003] ; Government Program of Competitive Growth of Kazan Federal University ; RUDN University program 5-100
WOS关键词	SOIL ORGANIC-MATTER ; LABILE CARBON ; ENZYME-ACTIVITIES ; PADDY SOIL ; MINERALIZATION ; RICE ; BIOMASS ; GLUCOSE ; AVAILABILITY ; TURNOVER
WOS研究方向	Agriculture
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000518706800017
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; State Key Laboratory of Organic Geochemistry, GIGCAS ; Hunan Province Base for Scientific and Technological Innovation Cooperation ; Youth Innovation Team Project of Institute of Subtropical Agriculture, Chinese Academy of Sciences ; NSFC-RFBR joint project ; Innovation Groups of National Natural Science Foundation of Hunan Province ; Government Program of Competitive Growth of Kazan Federal University ; RUDN University program 5-100
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/133169]
专题	中国科学院地理科学与资源研究所
通讯作者	Ge, Tida
作者单位	1.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China 2.Yancheng Teachers Univ, Jiangsu Prov Key Lab Bioresources Coastal Saline, Jiangsu Coastal Biol Agr Synthet Innovat Ctr, Yancheng 224002, Peoples R China 3.RAS, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia 4.Univ La Frontera, Dept Ciencias Quim & Recursos Nat, Temuco, Chile 5.RUDN Univ, Agrotechnol Inst, Moscow 117198, Russia 6.Kazan Fed Univ, Inst Environm Sci, Kazan 420049, Russia 7.Univ Gottingen, Dept Agr Soil Sci, Dept Soil Sci Temperate Ecosyst, Gottingen, Germany 8.Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2UW, Gwynedd, Wales 9.Chinese Acad Sci, Key Lab Ecosyst Network Observat & Modeling, Inst Geog Sci & Nat Resources Res, Beijing, Peoples R China 10.Chinese Acad Sci, Inst Subtrop Agr, Changsha Res Stn Agr & Environm Monitoring, Changsha 410125, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Cui, Jun,Zhu, Zhenke,Xu, Xingliang,et al. Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming[J]. SOIL BIOLOGY & BIOCHEMISTRY,2020,142:13.
APA	Cui, Jun.,Zhu, Zhenke.,Xu, Xingliang.,Liu, Shoulong.,Jones, Davey L..,...&Ge, Tida.(2020).Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming.SOIL BIOLOGY & BIOCHEMISTRY,142,13.
MLA	Cui, Jun,et al."Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming".SOIL BIOLOGY & BIOCHEMISTRY 142(2020):13.