Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale

doi:10.1016/j.ecoenv.2020.110215

	Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale
	Wang, Ziquan 1,5; Tian, Haixia 5; Lei, Mei 6; Megharaj, Mallavarapu 2; Tan, Xiangping 3,5; Wang, Fang 1,4; Jia, Hanzhong 5; He, Wenxiang 5
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2020-03-15
卷号	191 页码:8
关键词	Field soil pollution Catalytic efficiency Risk assessment Bioindicator Ecological dose
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2020.110215
通讯作者	Jia, Hanzhong(jiahz@nwafu.edu.cn) ; He, Wenxiang(wenxiang.he@nwafu.edu.cn)
英文摘要	Information on the kinetic characteristics of soil enzymes under long-term arsenic (As) pollution in field soils is scarce. We investigated Michaelis-Menten kinetic properties of four soil enzymes including beta-glucosidase (BG), acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenase (DHA) in field soils contaminated by As resulting from long-term realgar mining activity. The kinetic parameters, namely the maximum reaction velocity (V-max), enzyme-substrate affinity (K-m) and catalytic efficiency (V-max/K-m) were calculated. Results revealed that the enzyme kinetic characteristics varied in soils and were significantly influenced by total nitrogen (N) and total As, which explained 31.8% and 30.7% of the variance in enzyme kinetics respectively. Enzyme pools (V-max) and catalytic efficiency (V-max/K-m) of BG, ACP and DHA decreased with elevated As pollution, while the enzyme affinity for substrate (K-m) was less affected. Redundancy analysis and stepwise regression suggested that the adverse influence of As on enzyme kinetics may offset or weakened by soil total N and soil organic matter (SOM). Concentration-response fitting revealed that the specific kinetic parameters expressed as the absolute enzyme kinetic parameters multiplied by normalized soil total N and SOM were more relevant than the absolute ones to soil total As. The arsenic ecological dose values that cause 10% decrease (ED10) in the specific enzyme kinetics were 20-49 mg kg(-1), with a mean value of 35 mg kg(-1), indicating a practical range of threshold for As contamination at field level. This study concluded that soil enzymes exhibited functional adaptation to long-term As stress mainly through the reduction of enzyme pools (Vmax) or maintenance of enzyme-substrate affinity (K-m). Further, this study demonstrates that the specific enzyme kinetics are the better indicators of As ecotoxicity at field-scale compared with the absolute enzyme parameters.
资助项目	National Natural Science Foundation of China[41571245] ; National Natural Science Foundation of China[41603116] ; National Natural Science Foundation of China[31600384] ; Basic Scientific Research Foundation of Northwest A F University[ZD2013012] ; China Postdoctoral Science Foundation[2016M592549]
WOS关键词	ALKALINE-PHOSPHATASE ; MICROBIAL COMMUNITY ; DEHYDROGENASE-ACTIVITY ; CATALYTIC EFFICIENCY ; HEAVY-METALS ; CONTAMINATION ; TOXICITY ; AVAILABILITY ; REMEDIATION ; MANAGEMENT
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000514011400011
资助机构	National Natural Science Foundation of China ; Basic Scientific Research Foundation of Northwest A F University ; China Postdoctoral Science Foundation
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/132834]
专题	中国科学院地理科学与资源研究所
通讯作者	Jia, Hanzhong; He, Wenxiang
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China 2.Univ Newcastle, Fac Sci, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia 3.Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, 723 Xingke Rd, Guangzhou 510650, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Northwest A&F Univ, Coll Nat Resources & Environm, Key Lab Plant Nutr & Agroenvironm Northwest China, Minist Agr, Yangling 712100, Shaanxi, Peoples R China 6.Chinese Acad Sci, Ctr Environm Remediat, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ziquan,Tian, Haixia,Lei, Mei,et al. Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,191:8.
APA	Wang, Ziquan.,Tian, Haixia.,Lei, Mei.,Megharaj, Mallavarapu.,Tan, Xiangping.,...&He, Wenxiang.(2020).Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,191,8.
MLA	Wang, Ziquan,et al."Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 191(2020):8.