Establishment of a chloroplast transformation system inTisochrysis lutea

doi:10.1007/s10811-020-02159-4

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Establishment of a chloroplast transformation system inTisochrysis lutea
	Bo, Yuntai 5; Wang, Kang 4; Wu, Yuanyuan 5; Cao, Haiyang 5; Cui, Yulin 3; Wang, Lan 1,2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2020-07-14
页码	7
关键词	Tisochrysis lutea Genetic transformation Chloroplast Biolistic method
ISSN号	0921-8971
DOI	10.1007/s10811-020-02159-4
通讯作者	Wang, Lan(WL_312@163.com)
英文摘要	Tisochrysis luteais a haptophyte microalga commonly used as a commercial feed for juvenile fish and shellfish larvae. Genetic modification is of considerable importance for developing the potential economic value ofT. lutea. However, the genetic transformation system ofT. luteahas not yet been established, which limits functional genomic studies and strain improvement of this important microalgal species. In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 andant2) driven by the endogenouspsbAandrbcLpromoters were cloned in this vector. The recombinant plasmid was transformed using a biolistic method into thetrnI/trnAlocus of the chloroplast genome via homologous recombination. After continuous selection on phosphinothricin, the integration of foreign genes and the expression of specific products in the transformants were detected using polymerase chain reaction (PCR), Southern blotting, and western blot analysis. This is the first report of establishment of a stable transformation system inT. lutea, which is a prerequisite for functional genomics and applied research on this species.
资助项目	National Natural Science Foundation of China[41876188] ; Natural Science Foundation of Shandong Province, China[ZR2018ZB0210] ; Project of Innovation & Development of Marine Economy[HHCL201803]
WOS关键词	CULTIVATION MODE ; CHLAMYDOMONAS ; DNA
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000548460700001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province, China ; Project of Innovation & Development of Marine Economy
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28628]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Wang, Lan
作者单位	1.North China Sea Marine Forecasting Ctr SOA, 27 Yunling Rd, Qingdao 266000, Shandong, Peoples R China 2.Shandong Prov Key Lab Marine Ecol & Environm & Di, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China 4.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China 5.Yantai Marine Environm Monitoring Ctr Stn SOA, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Bo, Yuntai,Wang, Kang,Wu, Yuanyuan,et al. Establishment of a chloroplast transformation system inTisochrysis lutea[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:7.
APA	Bo, Yuntai,Wang, Kang,Wu, Yuanyuan,Cao, Haiyang,Cui, Yulin,&Wang, Lan.(2020).Establishment of a chloroplast transformation system inTisochrysis lutea.JOURNAL OF APPLIED PHYCOLOGY,7.
MLA	Bo, Yuntai,et al."Establishment of a chloroplast transformation system inTisochrysis lutea".JOURNAL OF APPLIED PHYCOLOGY (2020):7.