Advances in High-Speed Structured Illumination Microscopy | |
Zhao, Tianyu4,5,6; Wang, Zhaojun6; Chen, Tongsheng2,3; Lei, Ming4,6; Yao, Baoli4,5; Bianco, Piero R.1 | |
刊名 | FRONTIERS IN PHYSICS |
2021-05-28 | |
卷号 | 9 |
关键词 | fluorescence microscopy super-resolution SIM hardware acceleration of deep learning image reconstructed algorithm |
ISSN号 | 2296-424X |
DOI | 10.3389/fphy.2021.672555 |
产权排序 | 1 |
英文摘要 | Super-resolution microscopy surpasses the diffraction limit to enable the observation of the fine details in sub-cellular structures and their dynamics in diverse biological processes within living cells. Structured illumination microscopy (SIM) uses a relatively low illumination light power compared with other super-resolution microscopies and has great potential to meet the demands of live-cell imaging. However, the imaging acquisition and reconstruction speeds limit its further applications. In this article, recent developments all targeted at improving the overall speed of SIM are reviewed. These comprise both hardware and software improvements, which include a reduction in the number of raw images, GPU acceleration, deep learning and the spatial domain reconstruction. We also discuss the application of these developments in live-cell imaging. |
语种 | 英语 |
出版者 | FRONTIERS MEDIA SA |
WOS记录号 | WOS:000660058400001 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/94894] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Lei, Ming; Bianco, Piero R. |
作者单位 | 1.Univ Nebraska Med Ctr, Coll Pharm, Dept Pharmaceut Sci, Omaha, NE 68198 USA 2.South China Normal Univ, Coll Biophoton, Guangdong Prov Key Lab Laser Life Sci, Guangzhou, Peoples R China 3.South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou, Peoples R China 4.Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing, Peoples R China 5.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China 6.Xi An Jiao Tong Univ, Sch Phys, MOE Key Lab Nonequilibrium Synth & Modulat Conden, Xian, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Tianyu,Wang, Zhaojun,Chen, Tongsheng,et al. Advances in High-Speed Structured Illumination Microscopy[J]. FRONTIERS IN PHYSICS,2021,9. |
APA | Zhao, Tianyu,Wang, Zhaojun,Chen, Tongsheng,Lei, Ming,Yao, Baoli,&Bianco, Piero R..(2021).Advances in High-Speed Structured Illumination Microscopy.FRONTIERS IN PHYSICS,9. |
MLA | Zhao, Tianyu,et al."Advances in High-Speed Structured Illumination Microscopy".FRONTIERS IN PHYSICS 9(2021). |
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