Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation

doi:10.1016/j.enzmictec.2020.109531

	Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation
	Zhang, Jun 1,3; Xu, Chao 1,3; Chen, Xiaoyan 1,3; Ruan, Xi 4; Zhang, Yu 1; Xu, Huijuan 1; Guo, Ying 1; Xu, Jingliang 1,2; Lv, Pengmei 1; Wang, Zhongming 1
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2020-05-01
卷号	136 页码:8
关键词	D-Psicose D-Tagatose 3-epimerase Bacillus subtilis Fermentation Wasted apple hydrolysate
ISSN号	0141-0229
DOI	10.1016/j.enzmictec.2020.109531
通讯作者	Xu, Jingliang(xujl@zzu.edu.cn)
英文摘要	D-Psicose, as important rare sugar and epimer of D-fructose on the C-3 position, displays unique health benefits and physiological functions in various fields. The production of D-psicose is currently obtained via enzymatic bioconversion, mostly with of D-tagatose 3-epimerase. In the present study, the isomerization of D-fructose into D-psicose was achieved by constructing engineered food-grade Bacillus subtilis through fermentation. After optimizing the fermentation conditions of the recombinant strain, the yield of D-psicose reached up to 4.56 g/L, representing an over 8-fold increase in the yield compared to unoptimized conditions, with a D-fructose conversion rate of 56.26 % when the strain was cultured at 35 degrees C for 24 h with 5 g/L D-fructose and 5mM of Mn2+. In addition, 30 g of wasted apple hydrolysate were fermented by engineered B. subtilis and gained 7.76 g/L of D-psicose at a conversion rate of 25.86 % (w/w).
资助项目	National Key R&D Program of China[2018YFB1501701] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] ; Key Project on International Cooperation of Chinese Academy of Sciences[182344KYSB20160056] ; Guangdong Province Industrial High-tech Sector Science and Technology Project[2017A010105018] ; Guangdong Special Support Program[2017TX04Z109] ; Zhengzhou Science and Technology Innovation Leading Talent Project
WOS关键词	HYDROGEN-PRODUCTION ; METAL-IONS ; STRATEGY ; GLUCOSE ; EXPRESSION ; BIOMASS ; OXYGEN
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000528248400004
资助机构	National Key R&D Program of China ; Municipal Science and Technology Project of Guangzhou (China) ; Key Project on International Cooperation of Chinese Academy of Sciences ; Guangdong Province Industrial High-tech Sector Science and Technology Project ; Guangdong Special Support Program ; Zhengzhou Science and Technology Innovation Leading Talent Project
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/26700]
专题	中国科学院广州能源研究所
通讯作者	Xu, Jingliang
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 3.Univ China Acad Sci, Beijing 100049, Peoples R China 4.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jun,Xu, Chao,Chen, Xiaoyan,et al. Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation[J]. ENZYME AND MICROBIAL TECHNOLOGY,2020,136:8.
APA	Zhang, Jun.,Xu, Chao.,Chen, Xiaoyan.,Ruan, Xi.,Zhang, Yu.,...&Wang, Zhongming.(2020).Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation.ENZYME AND MICROBIAL TECHNOLOGY,136,8.
MLA	Zhang, Jun,et al."Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation".ENZYME AND MICROBIAL TECHNOLOGY 136(2020):8.