Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production

doi:10.1007/s10528-019-09906-4

	Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production
	Zhang, Yu 2,3,4; Zhang, Xiaohuan 2,3,4,5; Xiao, Shiyuan 2,3,4,5; Qi, Wei 2,3,4; Xu, Jingliang 1; Yuan, Zhenhong 2,3,4; Wang, Zhongming 2,3,4
刊名	BIOCHEMICAL GENETICS
	2019-06-01
卷号	57 期号:3 页码:443-454
关键词	3-Methyl-1-butanol L-Leucine Corynebacterium glutanicum Mutation breeding Non-fermentation pathway
ISSN号	0006-2928
DOI	10.1007/s10528-019-09906-4
通讯作者	Qi, Wei(qiwei@ms.giec.ac.cn) ; Xu, Jingliang(xujl@zzu.edu.cn)
英文摘要	3-Methyl-1-butanol (3MB) is a promising biofuel that can be produced from 2-ketoisocaproate via the common l-leucine biosynthesis pathway. Corynebacterium glutamicum was chosen as a host bacterium because of its strong resistance to isobutanol. In the current study, several strategies were designed to overproduce 3MB in C. glutamicum through a non-fermentation pathway. The engineered C. glutamicum mutant was obtained by silencing the pyruvate dehydrogenase gene complex (aceE) and deleting the lactic dehydrogenase gene (ldh), followed by mutagenesis with diethyl sulfate (DES) and selection with Fmoc-3-4-thiazolyl-l-alanine (FTA). The mutant could produce 659 mg/L of 3MB after 12 h of incubation. To facilitate carbon flux to 3MB biosynthesis, the engineered recombinant was also constructed without branched-chain acid aminotransferase (ilvE) activity by deleting the ilvE gene. This recombinant could produce 697 mg/L of 3MB after 12 h of incubation.
资助项目	National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[21811530626] ; Municipal Science and Technology Program of Guangzhou (China)[201804010081]
WOS关键词	AMINO-ACIDS ; BIOSYNTHESIS ; ISOBUTANOL ; PLASMIDS ; CLONING
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	SPRINGER/PLENUM PUBLISHERS
WOS记录号	WOS:000473825900006
资助机构	National Natural Science Foundation of China ; Municipal Science and Technology Program of Guangzhou (China)
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/25287]
专题	中国科学院广州能源研究所
通讯作者	Qi, Wei; Xu, Jingliang
作者单位	1.Zhengzhou Univ, Sch Chem Engn & Energy, Zhengzhou 450001, Henan, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yu,Zhang, Xiaohuan,Xiao, Shiyuan,et al. Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production[J]. BIOCHEMICAL GENETICS,2019,57(3):443-454.
APA	Zhang, Yu.,Zhang, Xiaohuan.,Xiao, Shiyuan.,Qi, Wei.,Xu, Jingliang.,...&Wang, Zhongming.(2019).Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production.BIOCHEMICAL GENETICS,57(3),443-454.
MLA	Zhang, Yu,et al."Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production".BIOCHEMICAL GENETICS 57.3(2019):443-454.