Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method | |
Zhao, Cheng1,2,3,4; Luo, Mu-Tan1,2,3,4; Huang, Chao1,2,3,5; Chen, Xue-Fang1,2,3,5; Xiong, Lian1,2,3,5; Li, Hai-Long1,2,3,5; Chen, Xin-De1,2,3,5 | |
刊名 | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY |
2019-03-28 | |
页码 | 9 |
关键词 | Fluorescent method microbial lipid Nile Red oleaginous yeasts |
ISSN号 | 1082-6068 |
DOI | 10.1080/10826068.2019.1587624 |
通讯作者 | Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com) |
英文摘要 | A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 degrees C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 mu g/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R-2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production. |
资助项目 | National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Youth Innovation Promotion Association CAS[2015290] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001] |
WOS关键词 | MICROALGAE ; BIODIESEL ; FEEDSTOCKS ; VARIETY |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | TAYLOR & FRANCIS INC |
WOS记录号 | WOS:000465953000001 |
资助机构 | National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Youth Innovation Promotion Association CAS ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.giec.ac.cn/handle/344007/24855] |
专题 | 中国科学院广州能源研究所 |
通讯作者 | Huang, Chao; Chen, Xin-De |
作者单位 | 1.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Cheng,Luo, Mu-Tan,Huang, Chao,et al. Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2019:9. |
APA | Zhao, Cheng.,Luo, Mu-Tan.,Huang, Chao.,Chen, Xue-Fang.,Xiong, Lian.,...&Chen, Xin-De.(2019).Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,9. |
MLA | Zhao, Cheng,et al."Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2019):9. |
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