Identification of two genes potentially related to myogenesis and muscle growth in Fenneropenaeus chinensis: Activin receptor II and Follistatin-like protein

doi:10.1016/j.gene.2020.145346

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	Identification of two genes potentially related to myogenesis and muscle growth in Fenneropenaeus chinensis: Activin receptor II and Follistatin-like protein
	Yan, Yunjun 1,2; Kong, Jie 1,2; Lu, Xia 2,3; Meng, Xianhong 2; Luan, Sheng 2; Dai, Ping 2; Chen, Baolong 2; Cao, Baoxiang 2; Luo, Kun 2
刊名	GENE
	2021-02-20
卷号	770 页码:16
关键词	Fenneropenaeus chinensis Activin receptor Follistatin-like protein Myogenesis Muscle growth RNAi
ISSN号	0378-1119
DOI	10.1016/j.gene.2020.145346
通讯作者	Kong, Jie(aquagene@163.com) ; Lu, Xia(xialu@yic.ac.cn)
英文摘要	Activin receptor (ActR) and follistatin-like (FSTL) genes, which are involved in the Myostatin (Mstn) related TGF-beta/Smad signaling pathway, play important roles in regulating the muscle generation, development and growth of muscle in vertebrate. Our previous studies have confirmed that Mstn negatively regulates muscle development and growth in Fenneropenaeus chinensis as that in vertebrate. However, the roles of ActR and FSTL in muscle development and growth in invertebrate remains unclear. In the present study, type II ActR(FcActRII) and FSTL (FcFSTL) genes from F. chinensis were cloned and characterized, and their functions on muscle development and growth were investigated. The full-length cDNAs of FcActRII and FcFSTL were 2366 bp that encoded 572 amino acids and 2474 bp that encoded 717 amino acids, respectively. Sequence analysis revealed that the overall protein sequences of the two genes shared 97% and 96% identities with Penaeus vannamei and 50%-59% and 35%-36% identities with vertebrates, respectively. In the early development stages, muscles firstly appeared in nauplius stage and developed gradually until post larval, and the mRNA expressions of FcActRII increased from gastrula to zoea stage and then decreased from zoea stage to post larval stage while that of FcFSTL was lowest in gastrula stage and increased rapidly in nauplius stage and then expressed stably from nauplius stage to post-larval stage. In the adult shrimp, the two genes were widely distributed in the examined tissues. The FcActRII expression in muscle of L group was significantly lower than that of S group, but the FcFSTL expression showed an opposite result. After down-regulating the expression of FcMstn by RNAi, FcActRII expression was significantly down-regulated while that of FcFSTL was up-regulated. The present study suggested that FcActRII and FcFSTL, regulated by FcMstn, might be involved in myogenesis and muscle growth.
资助项目	National Key Research and Development Program of China[2018YFD0900103] ; National Natural Science Foundation of China[U1706203] ; National Natural Science Foundation of China[41676148] ; Central Public interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institute, CAFS[20603022017001] ; China Agriculture Research System-48
WOS关键词	MOLT-INHIBITING HORMONE ; CRUSTACEAN HYPERGLYCEMIC HORMONE ; MOLECULAR CHARACTERIZATION ; IMMUNOGLOBULIN SUPERFAMILY ; EXPRESSION ANALYSIS ; RNA INTERFERENCE ; BINDING-PROTEINS ; MYOSTATIN GENE ; BLUE-CRAB ; CLONING
WOS研究方向	Genetics & Heredity
语种	英语
WOS记录号	WOS:000609086200003
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Central Public interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institute, CAFS ; China Agriculture Research System-48
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27501]
专题	海岸带生物资源高效利用研究与发展中心
通讯作者	Kong, Jie; Lu, Xia
作者单位	1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yan, Yunjun,Kong, Jie,Lu, Xia,et al. Identification of two genes potentially related to myogenesis and muscle growth in Fenneropenaeus chinensis: Activin receptor II and Follistatin-like protein[J]. GENE,2021,770:16.
APA	Yan, Yunjun.,Kong, Jie.,Lu, Xia.,Meng, Xianhong.,Luan, Sheng.,...&Luo, Kun.(2021).Identification of two genes potentially related to myogenesis and muscle growth in Fenneropenaeus chinensis: Activin receptor II and Follistatin-like protein.GENE,770,16.
MLA	Yan, Yunjun,et al."Identification of two genes potentially related to myogenesis and muscle growth in Fenneropenaeus chinensis: Activin receptor II and Follistatin-like protein".GENE 770(2021):16.