海蜇幼体环介导等温扩增快速检测方法的建立和应用

doi:DOI:10.3969/J.ISSN.2095-4972.2017.04.010

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	海蜇幼体环介导等温扩增快速检测方法的建立和应用
	孙婷婷 ; 刘中援 ; 梁丽琨 ; 董志军
刊名	应用海洋学学报
	2017-11-15
卷号	36 期号:4 页码:538-544
关键词	marine biology Rhopilema esculentum loop-mediated isothermal amplification method rapid detection polyps 海洋生物学海蜇环介导等温扩增快速检测螅状幼体
ISSN号	2095-4972
DOI	DOI:10.3969/J.ISSN.2095-4972.2017.04.010
其他题名	Development a rapid assay to detect the edible jellyfish Rhopilema esculentum larvae using loop-mediated isothermal amplification method
产权排序	(1) 烟台大学生命科学学院 ; (2) 中国科学院烟台海岸带研究所
文献子类	Article
英文摘要	It is difficult to identify the scyphomedusae larvae by traditional morphological methods,due to the complex morphological characteristics in different stages of their life cycle. For early identifying and detecting of this species,a simple and effective method using the loop-mediated isothermal amplification method (LAMP) has been developed. LAMP primers of Rhopilema esculentum were designed based on the mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene. The amplification conditions,including the betaine and dNTPs concentrations,the inner primer to outer primer concentration ratio,reaction time and temperature,were optimized. The specificity,sensitivity and repeatability of the method were also tested. Our results show that the primers had specificity and repeatability. Samples from different geographic populations of R. esculentum and polyps produced positive results,while the other non-target scyphomedusae species produced negative results. In addition,the LAMP assay was more sensitive than conventional PCR. The detection limit of LAMP method was 0. 1 ng DNA. Therefore,the established LAMP assay is a sensitive,specific,fast,and easily performed method for detection of the edible jellyfish R. esculentum.
WOS关键词	Oceanography
WOS研究方向	Oceanography ; 海洋学
语种	中文
CSCD记录号	CSCD:6118554
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24274]
专题	海岸带生物资源高效利用研究与发展中心
作者单位	1.烟台大学生命科学学院; 2.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	孙婷婷,刘中援,梁丽琨,等. 海蜇幼体环介导等温扩增快速检测方法的建立和应用[J]. 应用海洋学学报,2017,36(4):538-544.
APA	孙婷婷,刘中援,梁丽琨,&董志军.(2017).海蜇幼体环介导等温扩增快速检测方法的建立和应用.应用海洋学学报,36(4),538-544.
MLA	孙婷婷,et al."海蜇幼体环介导等温扩增快速检测方法的建立和应用".应用海洋学学报 36.4(2017):538-544.