田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究
武敏; 秦松; 李莉莉; 常秀莲
刊名中国食品添加剂
2017-07-17
期号07页码:49-55
关键词菊糖 低聚果糖 分离 田口方法 优化
ISSN号1006-2513
其他题名Optimization of inulin-derived oligofructose standard monomer using Taguchi method
产权排序(1) 烟台大学生命科学学院 ; (2) 中国科学院烟台海岸带研究所
文献子类期刊论文
英文摘要On the current Fos standard products market,it only has GFn oligomer standard products but lack of Fn oligomer standard products. The separation conditions for Fn oligofructose monomer from fructooligosaccharides mixtures derived from inulooligosaccharides were optimized by the Taguchi method in this study. The results showed that the sample concentration was the most significant factor for inulin separation. The optimum separation could be obtained at flow rate 0.05 m L/min,sample concentration 100 g/L,sample volume 2 m L and elution solution of 5% ethanol with the column of Bio-Gel-P4(2.0×100 cm). The sample then submitted to Bio-Gel-P2(2.0×100 cm)column chromatography,the optimum separation conditions were: flow rate 0.05 m L/min,sample concentration 100 g/L,sample volume 1 m L and elution solution of 5% ethanol. Under the above optimum conditions,the product from Bio-Gel-P4 column was concentrated and separated by Bio-Gel-P2 column chromatography. The purity of F3 was ≥ 98%, and F4 was ≥ 90%
语种中文
资助机构国家自然科学基金(21306221) ; 山东省科技重大专项(重大关键技术)(2015ZDJS03002) ; 烟台大学研究生科技创新基金资助(YDYB1716)
内容类型期刊论文
源URL[http://ir.yic.ac.cn/handle/133337/25208]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.烟台大学生命科学学院;
2.中国科学院烟台海岸带研究所
推荐引用方式
GB/T 7714
武敏,秦松,李莉莉,等. 田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究[J]. 中国食品添加剂,2017(07):49-55.
APA 武敏,秦松,李莉莉,&常秀莲.(2017).田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究.中国食品添加剂(07),49-55.
MLA 武敏,et al."田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究".中国食品添加剂 .07(2017):49-55.
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