产纤维素酶真菌分离及高效菌株筛选

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	产纤维素酶真菌分离及高效菌株筛选
	邵帅 ; 王玲玲 ; 林剑 ; 徐世艾 ; 李岩
刊名	河南农业科学
	2020-02-27
卷号	49 期号:04 页码:55-61
关键词	纤维素酶真菌鉴定优化酶活性
ISSN号	1004-3268
其他题名	Isolation of Cellulase-producing Fungi and Screening of Highly Efficient Strains
产权排序	(1) 烟台大学生命科学学院 ; (2) 中国科学院烟台海岸带研究所
文献子类	期刊论文
英文摘要	In order to isolate the highly efficient cellulose-degrading strain,a total of 24 fungi isolates were obtained from the decomposing fruit tree branches by using the dilution isolation method,with sodium carboxymethyl cellulose as the sole carbon substrate.Eight fungi strains were found to produce extracellular cellulase.Strains with larger hydrolytic circle/colony diameter in preliminary tests were selected to detect their celluase activities,and the results indicated strain 10 with highest activities.Its filter paper enzyme activity,endonuclease activity and exonuclease activity reached 13.26,36.67 and 12.06 IU respectively.Based on molecular and phylogenetic analyses,the strain was identified as Aspergillus terreus.Next the fermentation conditions through single factor optimizing were improved,and the optimal culture conditions for the three enzymes production were pH=7,the sodium carboxymethyl cellulose content=1.25%,and the sodium nitrate content=1.50%.After optimization,the filter paper enzyme activity,endonuclease activity and exonuclease activity reached 27.80,57.30,29.10 IU,which increased by 109.65%,56.26%,141.29% compared to pre-optimization,respectively.The highly efficient cellulase producing fungus strain 10 was provided for fruit branches fermentation and cellulase industrial development.
语种	中文
资助机构	国家自然科学基金项目(31600009) ; 中国科学院烟台海岸带研究所前沿部署项目(Y855021012) ; 吉林省与中科院科技合作高新技术产业化资金项目(2019SYHZ0036) ; 河北省重点研发计划项目(19273802D) ; 烟台市科技计划项目(2018ZHGY074)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25181]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.中国科学院烟台海岸带研究所 2.烟台大学生命科学学院;
推荐引用方式 GB/T 7714	邵帅,王玲玲,林剑,等. 产纤维素酶真菌分离及高效菌株筛选[J]. 河南农业科学,2020,49(04):55-61.
APA	邵帅,王玲玲,林剑,徐世艾,&李岩.(2020).产纤维素酶真菌分离及高效菌株筛选.河南农业科学,49(04),55-61.
MLA	邵帅,et al."产纤维素酶真菌分离及高效菌株筛选".河南农业科学 49.04(2020):55-61.