Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri

doi:10.1007/s00343-019-8014-3

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri
	Pang, YL ; Li, Y ; Liu, ZY ; Cui, YL ; Qin, S
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2019-03
卷号	37 期号:2 页码:651-656
关键词	green fluorescent protein (GFP) particle bombardment Sargassum horneri transgenesis
ISSN号	2096-5508
DOI	10.1007/s00343-019-8014-3
产权排序	[Pang Yunlong ; Liu Zhengyi ; Cui Yulin ; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China ; [Pang Yunlong] Univ Chinese Acad Sci, Beijing 101408, Peoples R China ; [Li Yan] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
文献子类	Article
英文摘要	Sargassum horneri is a macroalga widespread in North Asia-Pacific region, and these years its bloom has caused huge damage to the environment and the economic in China. To make up the blank on genetic engineering research, a transient transformation system for the multicellular marine brown alga S. horneri was established in this research. The algae used in this research were collected from the Yellow Sea of China and verified as a same species S. horneri with analysis of molecular markers. The S. horneri parietal leaves were transformed with the enhanced green fluorescent gene as the reporter by micro-particle bombardment. The results show that GFP is an effective transgene reporter for S. horneri and that particle bombardment is a suitable method for transformation of S. horneri. Through selection of four different promoters for EGFP and six groups' bombardment characters, the highest transformation efficiency approximately 1.31% was got with the vector pEGFP-N1 at bombardment characters 900 spi and 6 cm distance. This research paves a way for the further research and application of S. horneri.
WOS关键词	TRANSFORMATION ; CHLOROPHYTA ; PLASMIDS
WOS研究方向	Limnology ; Oceanography
语种	英语
WOS记录号	WOS:000464895600023
资助机构	National Key Research and Development Program of China [2016YFC1402106] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41406192, 41376139] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-023]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24982]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China; 3.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Pang, YL,Li, Y,Liu, ZY,et al. Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(2):651-656.
APA	Pang, YL,Li, Y,Liu, ZY,Cui, YL,&Qin, S.(2019).Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(2),651-656.
MLA	Pang, YL,et al."Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.2(2019):651-656.