FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

doi:10.1186/s12866-021-02138-w

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant
	Liu, Wei 1,2,3; Bai, Xue 1; Li, Yan 1,2,3; Zhang, Haikun 1,2,3; Hu, Xiaoke 1,2,3
刊名	BMC MICROBIOLOGY
	2021-03-09
卷号	21 期号:1 页码:9
关键词	Two-component response regulator REC domain HTH_LuxR domain Bacterial motility Exopolysaccharides Competitive nodulation
ISSN号	1471-2180
DOI	10.1186/s12866-021-02138-w
通讯作者	Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要	BackgroundA wide variety of bacterial adaptative responses to environmental conditions are mediated by signal transduction pathways. Two-component signal transduction systems are one of the predominant means used by bacteria to sense the signals of the host plant and adjust their interaction behaviour. A total of seven open reading frames have been identified as putative two-component response regulators in the gram-negative nitrogen-fixing bacteria Azorhizobium caulinodans ORS571. However, the biological functions of these response regulators in the symbiotic interactions between A. caulinodans ORS571 and the host plant Sesbania rostrata have not been elucidated to date.ResultsIn this study, we identified and investigated a two-component response regulator, AcfR, with a phosphorylatable N-terminal REC (receiver) domain and a C-terminal HTH (helix-turn-helix) LuxR DNA-binding domain in A. caulinodans ORS571. Phylogenetic analysis showed that AcfR possessed close evolutionary relationships with NarL/FixJ family regulators. In addition, six histidine kinases containing HATPase_c and HisKA domains were predicted to interact with AcfR. Furthermore, the biological function of AcfR in free-living and symbiotic conditions was elucidated by comparing the wild-type strain and the Delta acfR mutant strain. In the free-living state, the cell motility behaviour and exopolysaccharide production of the Delta acfR mutant were significantly reduced compared to those of the wild-type strain. In the symbiotic state, the Delta acfR mutant showed a competitive nodule defect on the stems and roots of the host plant, suggesting that AcfR can provide A. caulinodans with an effective competitive ability for symbiotic nodulation.ConclusionsOur results showed that AcfR, as a response regulator, regulates numerous phenotypes of A. caulinodans under the free-living conditions and in symbiosis with the host plant. The results of this study help to elucidate the involvement of a REC+HTH_LuxR two-component response regulator in the Rhizobium-host plant interaction.
资助项目	National Natural Science Foundation of China[31800099] ; National Natural Science Foundation of China[32070112]
WOS关键词	PROTEIN ; SYSTEM
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000627445700002
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27365]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Hu, Xiaoke
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wei,Bai, Xue,Li, Yan,et al. FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant[J]. BMC MICROBIOLOGY,2021,21(1):9.
APA	Liu, Wei,Bai, Xue,Li, Yan,Zhang, Haikun,&Hu, Xiaoke.(2021).FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant.BMC MICROBIOLOGY,21(1),9.
MLA	Liu, Wei,et al."FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant".BMC MICROBIOLOGY 21.1(2021):9.