Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk

doi:10.1021/acssensors.9b02205

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk
	Liu, Ping ; Li, Bowei ; Fu, Longwen ; Huang, Yan ; Man, Mingsan ; Qi, Ji ; Sun, Xiyan ; Kang, Qi ; Shen, Dazhong ; Chen, Lingxin
刊名	ACS SENSORS
	2020-02
卷号	5 期号:2 页码:464-473
关键词	INFRARED FLUORESCENT-PROBE HYDROGEN-SULFIDE NITRIC-OXIDE SULFANE SULFUR CANCER-CELLS CULTURE GRADIENTS SCAFFOLDS CHIP HNO
DOI	10.1021/acssensors.9b02205
产权排序	[Liu, Ping ; Li, Bowei ; Fu, Longwen ; Huang, Yan ; Man, Mingsan ; Qi, Ji ; Sun, Xiyan ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Liu, Ping ; Li, Bowei ; Fu, Longwen ; Huang, Yan ; Man, Mingsan ; Qi, Ji ; Sun, Xiyan ; Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Megasci, Yantai 264003, Peoples R China ; [Liu, Ping ; Kang, Qi ; Shen, Dazhong] Shandong Normal Univ, Key Lab Mol & Nano Probes, Minist Educ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China ; [Huang, Yan ; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	In this paper, we first proposed a novel hybrid three-dimensional (3D) printed and paper-based microfluidic platform and applied it for investigating the cell's apoptosis and intracellular cross-talk. The fabrication of a 3D-printed microfluidic chip is much easier than polydimethylsiloxane (PDMS) chip and can be applied in many common labs without soft lithogrophy fabrication equipment. Moreover, 3D printing can be perfectly combined with paper-based chips that can provide 3D scaffold for cell culture and analysis. In addition, these paper chips are disposable after use, greatly reducing the experimental cost. We integrated "Christmas Tree" structure with the top layer of the 3D-printed microfluidic chip to generate a continuous concentration gradient, and the bottom layer contained paper-based chips as cell culture area. The two-layer structure allows the concentration gradient forming layer to be separated from the cell culture layer, which can simplify the planting of cells in the microfluidic chip and make sure the cells stay in the culture chambers and don't clog the microfluidic channels. Applying this hybrid platform, we examined the effect of H2S on cancer cells. Continuous exposure to a low concentration of H2S inhibited cancer cell SMMC-7721 proliferation by inducing cell apoptosis. We also found that two gaseous molecules H2S and NO have cross-talk in cancer cells; they formed bioactive intermediate polysulfides in cancer cells. It is expected that this novel hybrid 3D-printed and paper-based microfluidic platform will have widespread application prospects in cell investigation.
WOS关键词	INFRARED FLUORESCENT-PROBE ; HYDROGEN-SULFIDE ; NITRIC-OXIDE ; SULFANE SULFUR ; CANCER-CELLS ; CULTURE ; GRADIENTS ; SCAFFOLDS ; CHIP ; HNO
语种	英语
WOS记录号	WOS:000517856700023
资助机构	National Key Research and Development Program of China [2016YFC1400702] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 21976209, 21874083, 81873697] ; Research Initiation Fund of Binzhou Medical University [BY2019KYQD39] ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars ; Taishan Scholar Project Special Funding [ts20190962]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25091]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_山东省海岸带环境工程技术研究中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 3.Shandong Normal Univ, Key Lab Mol & Nano Probes, Minist Educ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Megasci, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Ping,Li, Bowei,Fu, Longwen,et al. Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk[J]. ACS SENSORS,2020,5(2):464-473.
APA	Liu, Ping.,Li, Bowei.,Fu, Longwen.,Huang, Yan.,Man, Mingsan.,...&Chen, Lingxin.(2020).Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk.ACS SENSORS,5(2),464-473.
MLA	Liu, Ping,et al."Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk".ACS SENSORS 5.2(2020):464-473.