SERS-based droplet microfluidics for high-throughput gradient analysis

doi:10.1039/c8lc01180j

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	SERS-based droplet microfluidics for high-throughput gradient analysis
	Jeon, J ; Choi, N ; Chen, H ; Moon, JI ; Chen, LX ; Choo, J
刊名	LAB ON A CHIP
	2019-02-21
卷号	19 期号:4 页码:674-681
关键词	ENHANCED RAMAN-SCATTERING GOLD NANOFLOWERS IMMUNOASSAY PLATFORMS
ISSN号	1473-0197
DOI	10.1039/c8lc01180j
产权排序	[Jeon, Jinhyeok ; Choi, Namhyun ; Chen, Hao ; Moon, Joung-Il] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea ; [Chen, Hao ; Moon, Joung-Il ; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea ; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	In the last two decades, microfluidic technology has emerged as a highly efficient tool for the study of various chemical and biological reactions. Recently, we reported that high-throughput detection of various concentrations of a reagent is possible using a continuous gradient microfluidic channel combined with a surface-enhanced Raman scattering (SERS) detection platform. In this continuous flow regime, however, the deposition of nanoparticle aggregates on channel surfaces induces the memory effect, affecting both sensitivity and reproducibility. To resolve this problem, a SERS-based gradient droplet system was developed. Herein, the serial dilution of a reagent was achieved in a stepwise manner using microfluidic concentration gradient generators. Then various concentrations of a reagent generated in different channels were simultaneously trapped into the tiny volume of droplets by injecting an oil mixture into the channel. Compared to the single-phase regime, this two-phase liquid/liquid segmented flow regime allows minimization of resident time distributions of reagents through localization of reagents in encapsulated droplets. Consequently, the sample stacking problem could be solved using this system because it greatly reduces the memory effect. We believe that this SERS-based gradient droplet system will be of significant utility in simultaneously monitoring chemical and biological reactions for various concentrations of a reagent.
WOS关键词	ENHANCED RAMAN-SCATTERING ; GOLD NANOFLOWERS ; IMMUNOASSAY ; PLATFORMS
WOS研究方向	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000459726400011
资助机构	National Research Foundation of KoreaNational Research Foundation of Korea [2017M3D1A1039287, 2018M3A7B4071203] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 21575159]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24994]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; 3.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea;
推荐引用方式 GB/T 7714	Jeon, J,Choi, N,Chen, H,et al. SERS-based droplet microfluidics for high-throughput gradient analysis[J]. LAB ON A CHIP,2019,19(4):674-681.
APA	Jeon, J,Choi, N,Chen, H,Moon, JI,Chen, LX,&Choo, J.(2019).SERS-based droplet microfluidics for high-throughput gradient analysis.LAB ON A CHIP,19(4),674-681.
MLA	Jeon, J,et al."SERS-based droplet microfluidics for high-throughput gradient analysis".LAB ON A CHIP 19.4(2019):674-681.