Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe

doi:10.1039/c7tb03200e

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe
	Gao, Min ; Wang, Rui ; Yu, Fabiao ; Youc, Jinmao ; Chen, Lingxin
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2018-05-07
卷号	6 期号:17 页码:2608-2619
关键词	HYDROGEN POLYSULFIDES OXIDATIVE STRESS IN-VIVO CYSTEINE PERSULFIDES BIOLOGICAL-SYSTEMS CHEMICAL PROBES CANCER-CELLS SULFIDE CONSTRUCTION SUPEROXIDE
ISSN号	2050-750X
DOI	10.1039/c7tb03200e
产权排序	[Gao, Min ; Wang, Rui ; Yu, Fabiao ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yu, Fabiao ; Youc, Jinmao ; Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
文献子类	Article
英文摘要	Ischemia is a pathological condition owing to the deficiency of blood supply to a limited area of tissue. Ischemia can induce burst production of reactive oxygen species and lead to oxidative damage. As a family member of reactive sulfur species, sulfane sulfur plays important physiological roles in many biological events including synthesis of cofactors, modulation of enzyme activities, sulfuration of tRNA, and especially regulation of the intracellular redox state. We hypothesize that the endogenous level of sulfane sulfur will be adjusted to deal with ischemia-induced oxidative damage. Therefore, the bioimaging of sulfane sulfur real-time changes during ischemia is important for better understanding its physiological processes. Herein, we describe the development of a mitochondria-targeted fluorescent probe Mito-SH that allowed for selective and sensitive detection of sulfane sulfur. Mito-SH is designed on the basis of the tautomerization of sulfane sulfur to thiosulfoxide, which ensures its high selectivity and sensitivity. A lipophilic triphenylphosphonium cation is selected as the mitochondria-targeted moiety, which can precisely navigate Mito-SH into mitochondria. The emission profile of azo-BODIPY fluorophore locates at the near-infrared region, which deeply penetrates tissue and effectively avoids the interference of biological background. Mito-SH exhibits the desirable combination of selectivity, sensitivity and excellent fluorescence response upon reaction with sulfane sulfur in cells. By employing Mito-SH, we evaluate the real-time sulfane sulfur dynamic changes under oxygen-glucose deprivation. Finally, Mito-SH has been successfully used for imaging sulfane sulfur changes caused by acute ischemia in mice.
WOS关键词	HYDROGEN POLYSULFIDES ; OXIDATIVE STRESS ; IN-VIVO ; CYSTEINE PERSULFIDES ; BIOLOGICAL-SYSTEMS ; CHEMICAL PROBES ; CANCER-CELLS ; SULFIDE ; CONSTRUCTION ; SUPEROXIDE
WOS研究方向	Materials Science, Biomaterials
语种	英语
WOS记录号	WOS:000431421800013
资助机构	National Nature Science Foundation of China [21575159, 21775162, 41776110, 21405172] ; program of Youth Innovation Promotion Association, CAS [2015170] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] ; Instrument Developing Project of the CAS [YZ201662] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24465]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Min,Wang, Rui,Yu, Fabiao,et al. Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(17):2608-2619.
APA	Gao, Min,Wang, Rui,Yu, Fabiao,Youc, Jinmao,&Chen, Lingxin.(2018).Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe.JOURNAL OF MATERIALS CHEMISTRY B,6(17),2608-2619.
MLA	Gao, Min,et al."Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe".JOURNAL OF MATERIALS CHEMISTRY B 6.17(2018):2608-2619.