A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT

doi:10.1039/c7bm00951h

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT
	Han, Xiaoyue ; Song, Xinyu ; Li, Bowei ; Yu, Fabiao ; Chen, Lingxin
刊名	BIOMATERIALS SCIENCE
	2018-03-01
卷号	6 期号:3 页码:672-682
关键词	TURN-ON PROBE HYDROGEN-SULFIDE IN-VIVO CHEMICAL BIOLOGY OXIDATIVE STRESS RATIONAL DESIGN SYNTHASE CBS FAR-RED POLYSULFIDES CHEMISTRY
ISSN号	2047-4830
DOI	10.1039/c7bm00951h
产权排序	[Han, Xiaoyue ; Song, Xinyu ; Li, Bowei ; Yu, Fabiao ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yu, Fabiao ; Chen, Lingxin] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China
文献子类	Article
英文摘要	Sulfane sulfur refers to ionized sulfur that is reversibly attached to other sulfur atoms in the form of 6-valence electrons (S-0). Sulfane sulfur possesses stronger nucleophilicity and reducibility than hydrogen sulfide in a series of physiological reactions, which probably makes sulfane sulfur the actual signal molecule in cells. Herein, we designed and synthesized a near-infrared (NIR) fluorescent probe BD-diSH for sensitive detection and imaging of sulfane sulfur in living cells and in vivo. The probe BD-diSH is composed of two moieties: the fluorophore azo-BODIPY and the sulfane sulfur recognition unit, viz., 2-mercapto benzoate. BD-diSH displayed high sensitivity and selectivity towards sulfane sulfur. The mercapto group (-SH) of 2-mercapto benzoic acid can nucleophilically capture the sulfur atom of thiosulfoxide tautomers in sulfane sulfur to form -SSH. The group -SSH will immediately induce intramolecular cyclization reaction and release the azo-BODIPY fluorophore to emit NIR fluorescence. The probe BD-diSH was successfully applied to detect and image sulfane sulfur in the cytoplasm of the living cells. The results illustrated that the endogenous and exogenous sulfane sulfur level changed depending on different cell lines. BD-diSH was also capable of imaging the level changes of sulfane sulfur in mice. The above applications make our new probe a potential chemical tool for the study of physiological and pathological functions of sulfur sulfide in living cells and in vivo.
WOS关键词	TURN-ON PROBE ; HYDROGEN-SULFIDE ; IN-VIVO ; CHEMICAL BIOLOGY ; OXIDATIVE STRESS ; RATIONAL DESIGN ; SYNTHASE CBS ; FAR-RED ; POLYSULFIDES ; CHEMISTRY
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000426392200021
资助机构	State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] ; National Nature Science Foundation of China [21775162, 21405172, 21575159] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705] ; program of Youth Innovation Promotion Association, CAS [2015170]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24385]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoyue,Song, Xinyu,Li, Bowei,et al. A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT[J]. BIOMATERIALS SCIENCE,2018,6(3):672-682.
APA	Han, Xiaoyue,Song, Xinyu,Li, Bowei,Yu, Fabiao,&Chen, Lingxin.(2018).A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT.BIOMATERIALS SCIENCE,6(3),672-682.
MLA	Han, Xiaoyue,et al."A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT".BIOMATERIALS SCIENCE 6.3(2018):672-682.