Effects of ammonia nitrogen on gill mitochondria in clam Ruditapes philippinarum

doi:10.1016/j.etap.2018.12.003

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Effects of ammonia nitrogen on gill mitochondria in clam Ruditapes philippinarum
	Cong, Ming ; Wu, Huifeng ; Cao, Tengfei ; Ji, Chenglong ; Lv, Jiasen
刊名	ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
	2019-01
卷号	65 页码:46-52
关键词	Ruditapes philippinarum Ammonia nitrogen Mitochondrial transmembrane potential ATPase Gill function Histological observation
ISSN号	1382-6689
DOI	10.1016/j.etap.2018.12.003
产权排序	[Cong, Ming ; Wu, Huifeng ; Cao, Tengfei ; Ji, Chenglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proce & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Peoples R China ; [Wu, Huifeng ; Ji, Chenglong] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China ; [Cao, Tengfei ; Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
文献子类	Article
英文摘要	Ammonia nitrogen exposure has been found to significantly increase the early apoptosis rates of gill cells, affect the contents of ATP and disturb expressions of calcium-related genes in clam Ruditapes philippinarum. Mitochondria are the centers for energy production, initiation of apoptosis and calcium signal regulation. It is hypothesized that gill mitochondrion is a target organelle for the ammonia nitrogen. Thus, ATP metabolism together with ATP-consuming functions would be interfered by ammonia exposure. In the present study, mitochondrial transmembrane potential (MTP), ATPase activities, gill functions in clearance and respiration, and histological changes were detected to characterize the effects of ammonia to the gill mitochondria in clam R. philippinarum. Results indicated that ammonia exposure led to significant decreases in MTP, Ca2+-ATPase activity and clearance rates. However, different concentrations of ammonia nitrogen induced different variations on H+,-ATPase activity and respiration rates. Histological observation revealed that subacute exposure of ammonia damaged the microstructure of gill tissues. Therefore, ammonia exposure dramatically damaged the normal structure and function of mitochondria, resulting in irreversible damage in energy formation and supply. In addition, it affected Ca2+ and K+ metabolism and inhibited food intake and respiration in clam R. philippinarum.
WOS关键词	SEAWATER ACIDIFICATION ; NEUROTOXICITY ; AQUACULTURE ; DEPLETION ; TOXICITY ; BAY
WOS研究方向	Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology
语种	英语
WOS记录号	WOS:000457814500008
资助机构	Natural Science Foundation of China [NSFC 41876135] ; National Key Basic Research Program of China [2015CB453303] ; Youth Innovation Promotion Association CAS [2015169]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24615]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; 2.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proce & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Cong, Ming,Wu, Huifeng,Cao, Tengfei,et al. Effects of ammonia nitrogen on gill mitochondria in clam Ruditapes philippinarum[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2019,65:46-52.
APA	Cong, Ming,Wu, Huifeng,Cao, Tengfei,Ji, Chenglong,&Lv, Jiasen.(2019).Effects of ammonia nitrogen on gill mitochondria in clam Ruditapes philippinarum.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,65,46-52.
MLA	Cong, Ming,et al."Effects of ammonia nitrogen on gill mitochondria in clam Ruditapes philippinarum".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 65(2019):46-52.