液质联用技术在测定浮游植物色素与粒级分类中的应用

doi:10.11840/j.issn.1001-6392.2018.02.005

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	液质联用技术在测定浮游植物色素与粒级分类中的应用
	张晶晶 ; 刘东艳 ; 孙慧慧 ; 王晓峰
刊名	海洋通报
	2018-04-15
卷号	37 期号:02 页码:158-164
关键词	黄海 Chl a 浮游植物特征色素粒级结构 UHPLC-MS/MS
ISSN号	1001-6392
DOI	10.11840/j.issn.1001-6392.2018.02.005
其他题名	Application of UHPLC-MS/MS in determination of the phytoplankton pigment and size fraction
产权排序	(1)中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室 ; (2)中国科学院大学 ; (3)鲁东大学资源与环境工程学院 ; (4)华东师范大学河口海岸学国家重点实验室
英文摘要	In order to explore the feasibility of applying ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) on pigment analysis and size fraction monitoring, seawater samples collected from 3 different water layers at 47 sites in the Yellow Sea were analyzed, it in terms of pigment concentrations acquired by ultra-high performance liquid chromatography (UHPLC) and UHPLC-MS/MS respectively and results got from the two ways were compared. No observious differences were observed from the result comparisons between the two methods for most cases although UHPLC-MS/MS were more sensitive, and the two methods were both suitable for pigment detection in the Yellow Sea. Based on the pigment results provided by UHPLC-MS/MS, the spatial distribution of chlorophyll a (Chl a) and size fraction were studied. The concentration of Chl a was much higher in the northeast of Yangtze River mouth and coastal waters of Shandong Peninsula, where micro phytoplankton was the dominant size fraction. The concentration of Chl a was much lower in the central Yellow Sea, and the contribution of nano-and pico-phytoplankton increased significantly in this region. Compared with previous studies, the absolute concentrations of Chl a and contribution of size fractions in our study showed some differences, which may be caused by the differences of investigation time, sites or analytical methods. Thus, more comparison study is still needed to further probe the reason behind these differences.
资助项目	Basic Special Program of Ministry of Science and Technology[2014FY210600] ; National Natural Science Foundation of China[41376121] ; Natural Science Foundation of Shandong Province[JQ201414]
语种	中文
CSCD记录号	CSCD:6233769
资助机构	科技基础性工作专项#2014FY210600 ; 国家自然科学基金#41376121 ; 山东省自然科学基金委杰出青年基金项目#JQ201414
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24333]
专题	烟台海岸带研究所_近岸生态与环境实验室
作者单位	1.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室; 2.中国科学院大学; 3.华东师范大学河口海岸学国家重点实验室 4.鲁东大学资源与环境工程学院;
推荐引用方式 GB/T 7714	张晶晶,刘东艳,孙慧慧,等. 液质联用技术在测定浮游植物色素与粒级分类中的应用[J]. 海洋通报,2018,37(02):158-164.
APA	张晶晶,刘东艳,孙慧慧,&王晓峰.(2018).液质联用技术在测定浮游植物色素与粒级分类中的应用.海洋通报,37(02),158-164.
MLA	张晶晶,et al."液质联用技术在测定浮游植物色素与粒级分类中的应用".海洋通报 37.02(2018):158-164.