Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis

doi:10.1021/acs.analchem.0c04512

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis
	Lu, Jiao 1; Li, Zan 1; Gao, Qing 1; Tan, Jiangkun 1; Sun, Zhiwei 1; Chen, Lingxin 1,2; You, Jinmao 1,2
刊名	ANALYTICAL CHEMISTRY
	2021-02-23
卷号	93 期号:7 页码:3426-3435
ISSN号	0003-2700
DOI	10.1021/acs.analchem.0c04512
通讯作者	Li, Zan(lilizanzan@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn) ; You, Jinmao(jmyou6304@163.com)
英文摘要	Phagocyte respiratory burst in immune responses generates enormous amounts of reactive oxygen species (ROS) to fulfill primary defense against neoplasia. However, the beneficial functions associated with ROS, especially the potent oxidant/nucleophile peroxynitrite, in an immunological process are still ambiguous. Herein, we report the construction and biological assessment of cyanine-based fluorescent biosensors, which are based on a nonoxidative strategy for peroxynitrite detection. The established nonoxidative strategy is composed of nucleophilic substitution and nanoaggregate formation initiated by peroxynitrite. The proposed nonoxidative strategy in this study could maintain cellular oxidative stress in the critical process of detection and preserve homeostasis of cell metabolism. The remarkable detection sensitivity, reaction selectivity, and spectral photostability of our biosensors enabled us to visualize endogenous peroxynitrite levels in immune-stimulated phagocytes. With the aid of basal peroxynitrite imaging in an acute peritonitis model, the visualization of peroxynitrite level variations in immune responses of tumorigenesis was accomplished assisted by our biosensors. It is envisioned that our strategy provides a promising tool for early tumor diagnosis and evaluation of tumor suppression in the process of immune responses without disturbing the functions of ROS signaling transduction.
资助项目	National Natural Science Foundation of China[21976105] ; National Natural Science Foundation of China[21976209] ; Youth Program of Natural Science Foundation of Shandong Province[ZR2020QB149]
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000623041000014
资助机构	National Natural Science Foundation of China ; Youth Program of Natural Science Foundation of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27368]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Li, Zan; Chen, Lingxin; You, Jinmao
作者单位	1.Qufu Normal Univ, Sch Chem & Chem Engn, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lu, Jiao,Li, Zan,Gao, Qing,et al. Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis[J]. ANALYTICAL CHEMISTRY,2021,93(7):3426-3435.
APA	Lu, Jiao.,Li, Zan.,Gao, Qing.,Tan, Jiangkun.,Sun, Zhiwei.,...&You, Jinmao.(2021).Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis.ANALYTICAL CHEMISTRY,93(7),3426-3435.
MLA	Lu, Jiao,et al."Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis".ANALYTICAL CHEMISTRY 93.7(2021):3426-3435.