A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

doi:10.3390/mi11020186

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院安徽光学精密机械研究所

	A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection
	朱灵( zhu ling)
刊名	Micromachines
	2020-02-11
卷号	11
关键词	in situ PCR molecular diagnosis microfluidic chip POC device ZIKV
DOI	10.3390/mi11020186
通讯作者	Yang, Ke(keyang@rntek.cas.cn) ; Zhu, Ling(zhul@aiofm.ac.cn)
英文摘要	The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C-3-system for the rapid extraction and detection of ZIKV RNA. The C-3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and "in situ PCR" (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for "in situ PCR", in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C-3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.
资助项目	Class C talent fund project of Chinese Academy of Sciences[CXJJ-19-C03] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ2018QN22] ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui[OMST201701] ; Key Research and Development Program of Anhui[1804a09020051] ; Key Research and Development Program of Anhui[1804c04020335] ; Key Research and Development Program of Anhui[201904c03020001] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489]
WOS关键词	NUCLEIC-ACID EXTRACTION ; WHOLE-BLOOD ; AMPLIFICATION ; DNA ; CAPTURE ; IDENTIFICATION ; ADSORPTION ; EFFICIENT ; VIRUS
WOS研究方向	Science & Technology - Other Topics ; Instruments & Instrumentation
语种	英语
出版者	MDPI
WOS记录号	WOS:000520181500075
资助机构	Class C talent fund project of Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui ; Key Research and Development Program of Anhui ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/92924]
专题	合肥物质科学研究院_中科院安徽光学精密机械研究所
通讯作者	朱灵( zhu ling)
推荐引用方式 GB/T 7714	Zhu L. A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection[J]. Micromachines,2020,11.
APA	朱灵.(2020).A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection.Micromachines,11.
MLA	朱灵."A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection".Micromachines 11(2020).