We present the systematic de Haas-van Alphen (dHvA) quantum oscillations studies on the recently discovered topological Dirac semimetal pyrite PtBi2 single crystals. Remarkable dHvA oscillations are emerged at a low field about 1.5 T. From the analyses of the dHvA oscillations, we extract the high quantum mobilities, light effective masses and phase shift factors for the Dirac fermions in pyrite PtBi2. From the angular dependence of the dHvA oscillations, we map out the topology of the Fermi surface. Furthermore, we identify two additional oscillation frequencies that are not probed by the SdH oscillations, which provides us with opportunities to further understand its Fermi surface topology. (C) 2019 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.