In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+

doi:10.1016/j.cclet.2018.04.026

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+
	Zhang, Mengge 1,3; Wen, Ming 1,3; Xiong, Ying 1,3; Zhang, Longhua 1,3; Tian, Changlin 1,2,3
刊名	CHINESE CHEMICAL LETTERS
	2018-10-01
卷号	29 期号:10 页码:1509-1512
关键词	Fluorescence lifetime imaging microscopy In cell analysis of conformation change Ferroprotin
ISSN号	1001-8417
DOI	10.1016/j.cclet.2018.04.026
通讯作者	Zhang, Longhua(zlhustc@ustc.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
英文摘要	Fluorescence microscopy, as a sensitive method to detect microenvironment of molecules, is widely used in protein conformation and dynamic studies in live cells. Fluorescence lifetime imaging microscopy (FLIM), which is independent of fluorophore concentrations, scattering and bleaching, is a suitable tool to analyze membrane proteins in a single cell. Ferroportin (FPN), a multi-ion exporter in vertebrates, was modulated by metal ions with unknown mechanism. Herein, we fused green fluorescence protein on C-terminal of FPN (FPN-eGFP) and applied fluorescence lifetime to monitor conformation changes of FPN in a live cell. The fluorescence lifetime distribution showed a shift to shorter lifetime upon Mn2+ treatment, suggesting a preference conformation of FPN in Mn2+ exposure. It is also observed that the lifetime (rather than intensity) measurement was not strongly influenced by laser power. The observed fluorescence lifetime changes of FPN-eGFP upon Mn2+ treatments indicated that extracellular metal ions can modulate FPN through conformation exchanges between several different states. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
资助项目	National Key R&D Program of China[2016YFA0400900] ; National Key R&D Program of China[2017YFA0505300] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201564]
WOS关键词	IRON EXPORTER ; SPATIAL-RESOLUTION ; TRANSPORTER ; HEPCIDIN ; HOMEOSTASIS ; EXPRESSION ; PROTEINS ; CLONING ; FLIM
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000449448200015
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/34396]
专题	合肥物质科学研究院_中科院强磁场科学中心
通讯作者	Zhang, Longhua; Tian, Changlin
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Mengge,Wen, Ming,Xiong, Ying,et al. In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1509-1512.
APA	Zhang, Mengge,Wen, Ming,Xiong, Ying,Zhang, Longhua,&Tian, Changlin.(2018).In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+.CHINESE CHEMICAL LETTERS,29(10),1509-1512.
MLA	Zhang, Mengge,et al."In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+".CHINESE CHEMICAL LETTERS 29.10(2018):1509-1512.