Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis

doi:10.1007/s13577-021-00541-z

	Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis
	Huang, Yuan 1,2; Zheng, Yabing 1,2; Shao, Xiying 1,2; Shi, Lei 1,2; Li, Guangliang 1,2; Huang, Ping 1,2
刊名	HUMAN CELL
	2021-05-17
关键词	TPT1-AS1 Cell proliferation Paclitaxel sensitivity Breast cancer CASP2
ISSN号	0914-7470
DOI	10.1007/s13577-021-00541-z
通讯作者	Zheng, Yabing(zhengyabingcas@tom.com)
英文摘要	Long non-coding RNAs (lncRNAs) are key modulators during cancer progression. Application of using lncRNA expression to evaluate patient prognosis and sensitivity to treatment is highly anticipated, yet the expression and mechanism of many lncRNAs remain unknown. Herein, we projected for the investigation of TPT1-AS1 function in breast cancer. TPT1-AS1 was assessed by bioinformatic analysis of publicly available datasets and quantitative real-time PCR (qRT-PCR). Cell sensitivity to paclitaxel and cell proliferation was measured by flow cytometry and CCK-8. Interaction among TPT1-AS1, microRNA (miRNA, miR)-3156-5p and Caspase 2 (CASP2) was studied by bioinformatic analysis, qRT-PCR, western blot as well as dual luciferase reporter assay. Herein, TPT1-AS1 was significantly diminished in breast cancer from publicly available datasets and our collected samples. In breast cancer cells, TPT1-AS1 overexpression repressed cell proliferation and sensitized breast cancer cells to paclitaxel. RegRNA 2.0 predicted a potential interaction between TPT1-AS1 and miR-3156-5p which was confirmed by qRT-PCR as well as dual luciferase reporter assay. CASP2, a proapoptotic gene, was corroborated to be targeted by miR-3156-5p. Meanwhile, TPT1-AS1 upregulated CASP2 in breast cancer cells, and its biological function was reversed by CASP2 knockdown. Collectively, TPT1-AS1 diminished cell proliferation and sensitized cells to chemotherapy by sponging miR-3156-5p and upregulating CASP2, acting as a biomarker for patients with breast cancer.
资助项目	Zhejiang Provincial Medical Science and Technology program[2016RCA003] ; Zhejiang Science and Technology program[2016C33199]
WOS关键词	GENE ; RESISTANCE ; EXPRESSION ; APOPTOSIS ; PROMOTES
WOS研究方向	Cell Biology
语种	英语
出版者	SPRINGER JAPAN KK
WOS记录号	WOS:000651333300002
资助机构	Zhejiang Provincial Medical Science and Technology program ; Zhejiang Science and Technology program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122164]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Yabing
作者单位	1.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Dept Breast Med Oncol, Hangzhou 310022, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Breast Med Oncol, 1 East Banshan Rd, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yuan,Zheng, Yabing,Shao, Xiying,et al. Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis[J]. HUMAN CELL,2021.
APA	Huang, Yuan,Zheng, Yabing,Shao, Xiying,Shi, Lei,Li, Guangliang,&Huang, Ping.(2021).Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis.HUMAN CELL.
MLA	Huang, Yuan,et al."Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis".HUMAN CELL (2021).