Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway

doi:10.1016/j.fct.2021.112029

	Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway
	Zhang, Wang 1; Li, Jing-Ya 2,3; Wei, Xiao-Chen 1; Wang, Qian 1; Yang, Ji-Yang 1; Hou, Huan 1; Du, Zi-Wei 1; Wu, Xin-An 1
刊名	FOOD AND CHEMICAL TOXICOLOGY
	2021-03-01
卷号	149
关键词	DBP PPAR alpha AMPK Lipid metabolism Liver function
ISSN号	0278-6915
DOI	10.1016/j.fct.2021.112029
通讯作者	Wu, Xin-An(wuxinan@boe.com.cn)
英文摘要	Phateacid esters (PAEs), such as dibutyl phthalate (DBP), have been widely used and human exposure results into serious toxic effects; such as the development of fatty liver disease. In the present study, SD rat models for in vivo study (normal and fatty liver model group) and hepatocytes for in vitro study (normal and abnormal lipid metabolism model group) were established to determine the effects of DBP on liver function and discover the possible mechanisms. Meanwhile, the peroxisome proliferator activated receptor (PPAR alpha) blocker, GW6471, with the Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) activator, AICAR, were applied in vitro study to clarify the role of PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway in the process. Results suggested that DBP could activate PPAR alpha signaling pathway and affected the protein expression of SREBP, FAS and GPAT to cause hyperlipidemia and abnormal liver function. DBP also could inhibit the phosphorylation and activation of AMPK to inhibit the decomposition and metabolism of lipids. Interestingly, the effects of DBP could be alleviated by GW6471 and AICAR. Our experimental results provide reliable evidence that DBP exposure could further induce liver lipid metabolism disorder and other hepatic toxicity through PPAR alpha/SREBP-1c/FAS/ GPAT/AMPK signal pathway.
资助项目	Anhui Province Scientific and Technological Key Project (China)[1301042217]
WOS研究方向	Food Science & Technology ; Toxicology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000632256600032
资助机构	Anhui Province Scientific and Technological Key Project (China)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/121065]
专题	中国科学院合肥物质科学研究院
通讯作者	Wu, Xin-An
作者单位	1.Hefei BOE Hosp, Dept Pharm, Hefei, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Dept Biol Phys, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wang,Li, Jing-Ya,Wei, Xiao-Chen,et al. Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway[J]. FOOD AND CHEMICAL TOXICOLOGY,2021,149.
APA	Zhang, Wang.,Li, Jing-Ya.,Wei, Xiao-Chen.,Wang, Qian.,Yang, Ji-Yang.,...&Wu, Xin-An.(2021).Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway.FOOD AND CHEMICAL TOXICOLOGY,149.
MLA	Zhang, Wang,et al."Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR alpha/SREBP-1c/FAS/GPAT/AMPK signal pathway".FOOD AND CHEMICAL TOXICOLOGY 149(2021).