Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR

doi:10.1016/j.bbamem.2020.183515

	Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR
	Yu, Lu 1; Fang, Wei 2,3; He, Yao 2,3; Cai, Wenguang 4; Wei, Wei 5; Tian, Changlin 1,2,3
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
	2021-02-01
卷号	1863
关键词	Mycobacterial ESX-1 secretion system Membrane protein EccE(1) Site-directed spin labeling Electron Paramagnetic Resonance Secondary structure Transmembrane topology
ISSN号	0005-2736
DOI	10.1016/j.bbamem.2020.183515
通讯作者	Yu, Lu(luyu@hmfl.ac.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
英文摘要	Protein EccE(1) is an essential component of the mycobacterial ESX-1 secretion system, which plays a crucial part in the process of virulence factors secretion, especially for pathogenic mycobacteria such as Mycobacterium tuberculosis. While EccE(1) was previously postulated to be the inner membrane pore-forming unit of a membrane complex through which substrates are transported, the structural properties of EccE(1) remains to be explored. In the present study, systematic Site-Directed Spin Labeling (SDSL) and Electron Paramagnetic Resonance (EPR) spectroscopic studies was carried out to reveal the secondary structure and transmembrane topology of the Nterminal Domain of EccE(1) protein (EccE(1)-NTD) from M. smegmatis in detergent micelles. EPR-based mobility and accessibility analysis of the R1 side chain for 64 residue positions of EccE(1) -NTD indicates that the transmembrane domain adopts two a-helices spanning Phe7-Cys30 and Leu36-Ile54. A tentative structural topology model of EccE(1) -NTD embedded in membrane is also suggested based on EPR spectroscopic data in this study, which will provide further insights into this protein and the ESX secretion systems of mycobacteria.
资助项目	Natural Science Foundation of China[21825703] ; Natural Science Foundation of China[U1732275] ; Natural Science Foundation of China[21927814] ; Natural Science Foundation of China[U1832181] ; Natural Science Foundation of China[31500611] ; National Key Research and Development Program of China[2019YFA0405600] ; Users with Excellence Program of Hefei Science Center, CAS[2018HSC-UE013] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE005] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE020] ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX003]
WOS关键词	VII SECRETION ; ESX-1
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000603419900018
资助机构	Natural Science Foundation of China ; National Key Research and Development Program of China ; Users with Excellence Program of Hefei Science Center, CAS ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/119956]
专题	中国科学院合肥物质科学研究院
通讯作者	Yu, Lu; Tian, Changlin
作者单位	1.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 5.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Yu, Lu,Fang, Wei,He, Yao,et al. Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2021,1863.
APA	Yu, Lu,Fang, Wei,He, Yao,Cai, Wenguang,Wei, Wei,&Tian, Changlin.(2021).Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1863.
MLA	Yu, Lu,et al."Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1863(2021).