Crystal structure of the WD40 domain of human PLRG1

doi:10.1016/j.bbrc.2020.11.057

	Crystal structure of the WD40 domain of human PLRG1
	Wang, Xiaoyang 1,2; Li, Yanjun 3; Dai, Haiming 4; Xu, Chao 1,2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2021
卷号	534
关键词	Spliceosome X-ray crystallography The nineteen complex WD40 repeats
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2020.11.057
通讯作者	Xu, Chao(xuchaor@ustc.edu.cn)
英文摘要	PLRG1 is a evolutionarily conserved protein in spliceosome and plays an important role in maintaining the integral part of the splicoeosme and its proper splicing. Here we solved the high resolution crystal structure of the WD40 domain of human PLRG1 by crystallography and compared our crystal structure with the cryo-EM structure of PLRG1 bound with other splicing factors. We found that two loops of the WD40 domain become resolved upon binding to the proteins within the spliceosome. Thus our work characterize the dynamic property of PLRG1 during the spliceosome assembly by presenting its apo structure. (C) 2020 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of China[31770806] ; Major/Innovative Program of the Development Foundation of the Hefei Center for Physical Science and Technology[2018CXFX007] ; Thousand Young Talent program
WOS关键词	SPLICEOSOME
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000607348500022
资助机构	National Natural Science Foundation of China ; Major/Innovative Program of the Development Foundation of the Hefei Center for Physical Science and Technology ; Thousand Young Talent program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/119889]
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, Chao
作者单位	1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, MOE Key Lab Membraneless Organelles & Cellular Dy, Hefei 230027, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 3.Univ Toronto, Struct Genom Consortium, 101 Coll St, Toronto, ON M5G 1L7, Canada 4.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoyang,Li, Yanjun,Dai, Haiming,et al. Crystal structure of the WD40 domain of human PLRG1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2021,534.
APA	Wang, Xiaoyang,Li, Yanjun,Dai, Haiming,&Xu, Chao.(2021).Crystal structure of the WD40 domain of human PLRG1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,534.
MLA	Wang, Xiaoyang,et al."Crystal structure of the WD40 domain of human PLRG1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 534(2021).