Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model

doi:10.1111/jcmm.15966

	Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model
	Xiao, Boduan 2; Ying, Chang 2; Chen, Yongyi 3; Huang, Fang 4; Wang, Binrong 2; Fang, Huiling 2; Guo, Wan 2; Liu, Tao 1; Zhou, Xiumei 2; Huang, Biao 2
刊名	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
	2020-10-14
关键词	CD55-TMn CEA doxorubicin hydrochloride liver cancer oncolytic virotherapy
ISSN号	1582-1838
DOI	10.1111/jcmm.15966
通讯作者	Wang, Yigang(wangyigang43@163.com)
英文摘要	Oncolytic adenovirus (OA) has attracted increasing attention due to their specific proliferation in tumour cells and resulting in lysis of tumour cells. To further improve the antitumour effect of OA, in this study, we combined CD55-TRAIL-IETD-MnSOD (CD55-TMn), a CEA-controlled OA constructed previously, and chemotherapy to investigate their synergistic effect and possible mechanisms. MTT assay was performed to detect antitumour effects. Hoechst 33 342 and flow cytometric analysis were used to examine cell apoptosis. Western blotting was performed to examine cell pyroptosis and apoptosis mechanism. Animal experiment was used to detect antitumour effect of doxorubicin hydrochloride (Dox) combined with CD55-TMn in vivo. We firstly found that Dox promotes gene expression mediated by CEA-regulated OA and virus progeny replication by activating phosphorylation of Smad3, and Dox can enhance antitumour effect of CEA-regulated CD55-TMn by promoting cell apotopsis and cell pyroptosis. Thus, our results provide an experimental and theoretical basis on tumour therapy by combination treatment of the oncolytic virotherapy and chemotherapy and it is expected to become a novel strategy for liver cancer therapy.
资助项目	Natural Science Foundation of Zhejiang Province[LY18C070002] ; National Nature Science Foundation of China[81803069] ; Grant for 521 talent project of ZSTU[2019A1515011678] ; Guangdong Provincial Natural Science Foundation of China[2019A1515011678]
WOS关键词	MANGANESE SUPEROXIDE-DISMUTASE ; GENE-THERAPY ; IN-VITRO ; ADENOVIRUS ; TRAIL ; COMBINATION ; OVEREXPRESSION ; APOPTOSIS ; XENOGRAFT ; RECEPTOR
WOS研究方向	Cell Biology ; Research & Experimental Medicine
语种	英语
出版者	WILEY
WOS记录号	WOS:000578244400001
资助机构	Natural Science Foundation of Zhejiang Province ; National Nature Science Foundation of China ; Grant for 521 talent project of ZSTU ; Guangdong Provincial Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/104666]
专题	中国科学院合肥物质科学研究院
通讯作者	Wang, Yigang
作者单位	1.Guangdong Gen Hosp, Guangdong Acad Med Sci, Dept Otolaryngol, Guangzhou, Peoples R China 2.Zhejiang Sci Tech Univ, Xinyuan Inst Med & Biotechnol, Sch Life Sci & Med, Hangzhou 310018, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Inst Canc Res & Basic Med Sci, Hangzhou, Peoples R China 4.Zhejiang Prov Peoples Hosp, Dept Pathol, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Boduan,Ying, Chang,Chen, Yongyi,et al. Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020.
APA	Xiao, Boduan.,Ying, Chang.,Chen, Yongyi.,Huang, Fang.,Wang, Binrong.,...&Wang, Yigang.(2020).Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE.
MLA	Xiao, Boduan,et al."Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020).