Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway

doi:10.3892/or.2020.7518

	Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway
	Xu, Shenglin 1; Wang, Yuan 1; Que, Yukang 1; Ma, Chengxiao 1; Cai, Shang 1; Wang, Hao 1; Yang, Xingyu 2; Yang, Chunjun 2; Cheng, Cheng 3; Zhao, Guoping 4
刊名	ONCOLOGY REPORTS
	2020-05-01
卷号	43
关键词	cold atmospheric plasma osteosarcoma mitochondrial reactive oxygen species apoptosis
ISSN号	1021-335X
DOI	10.3892/or.2020.7518
通讯作者	Hu, Yong(hy.in163@163.com)
英文摘要	The present study aimed to investigate the effects of cold atmospheric plasma (CAP)-activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP-activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin-V and propidium iodide. The expression levels of cytochrome c, caspase-3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC-1 dye method and verified by the level of reactive oxygen species (ROS). CAP-activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose- and time-dependent manner. Furthermore, CAP-activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP-activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS-mediated mitochondrial apoptosis.
资助项目	Natural Science Foundation Project of Anhui Province[1708085MH215] ; School Fund of Anhui Medical University[2015xkj088]
WOS关键词	PROGNOSTIC-FACTORS ; SURVIVAL ; CHEMOTHERAPY ; EXPERIENCE ; CHILDREN ; MEDIA ; HEAD ; PART
WOS研究方向	Oncology
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000526743200029
资助机构	Natural Science Foundation Project of Anhui Province ; School Fund of Anhui Medical University
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103655]
专题	中国科学院合肥物质科学研究院
通讯作者	Hu, Yong
作者单位	1.Anhui Med Univ, Dept Orthoped, Affiliated Hosp 1, 218 Jixi Rd, Hefei 230000, Anhui, Peoples R China 2.Anhui Med Univ, Dept Dermatol, Affiliated Hosp 2, Hefei 230000, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Plasma Phys, Hefei 230000, Anhui, Peoples R China 4.Chinese Acad Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei Inst Phys Sci, Hefei 230000, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xu, Shenglin,Wang, Yuan,Que, Yukang,et al. Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway[J]. ONCOLOGY REPORTS,2020,43.
APA	Xu, Shenglin.,Wang, Yuan.,Que, Yukang.,Ma, Chengxiao.,Cai, Shang.,...&Hu, Yong.(2020).Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway.ONCOLOGY REPORTS,43.
MLA	Xu, Shenglin,et al."Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway".ONCOLOGY REPORTS 43(2020).