Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase

	Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase
	Zhang, Xianlong; Li, Guoliang; Wu, Di; Yu, Yanxin; Hu, Na; Wang, Honglun; Li, Xiuting; Wu, Yongning
刊名	FOOD & FUNCTION
	2020
卷号	11 期号:1
英文摘要	The high incidence of diabetes mellitus has caused widespread concern around the world, and has quickly become one of the most prevalent and costly chronic diseases. Alpha-glucosidase has been considered as the main target enzyme in the prevention and treatment of type 2 diabetes, and the inhibition of alpha-glucosidase activity can control postprandial blood glucose levels of diabetics and keep blood glucose levels normal. However, most of the current antidiabetic drugs in the clinic have many side effects. Recently, the screening of alpha-glucosidase inhibitors, from natural products, with excellent activity and less side effects has been successfully achieved, which also proposes more requirements and challenges for the approaches of alpha-glucosidase inhibitor (AGI) screening because of the trace level of active compounds in natural products, large-scale sample screening, and complex matrix of natural products. In this review, we comprehensively summarized the available strategies for the assay of alpha-glucosidase activity and AGI screening for the first time, including classic strategies such as high throughput screening methods (e.g. capillary electrophoresis strategies, HPLC/LC-MS strategies), emerging sensing strategies (e.g. colorimetric sensing, fluorescence sensing, electrochemical sensing, and surface plasmon resonance sensing) and other strategies. Finally, future opportunities and challenges in future research are tentatively proposed.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60469]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Xianlong,Li, Guoliang,Wu, Di,et al. Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase[J]. FOOD & FUNCTION,2020,11(1).
APA	Zhang, Xianlong.,Li, Guoliang.,Wu, Di.,Yu, Yanxin.,Hu, Na.,...&Wu, Yongning.(2020).Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase.FOOD & FUNCTION,11(1).
MLA	Zhang, Xianlong,et al."Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase".FOOD & FUNCTION 11.1(2020).