Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis Through Inhibiting mTOR/AKT Signaling

	Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis Through Inhibiting mTOR/AKT Signaling
	Shan, Zhongshu; Bi, Hongtao; Suonan, Angxiu; Gu, Yong; Zhou, Huan; Xi, Kun; Xiong, Rui; Chen, Hua; Chen, Liang
刊名	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2020
卷号	15
关键词	tobacco mosaic virus viral nanoparticle osteoclast mTOR tibial bone injury
英文摘要	Introduction: Tobacco mosaic virus-based nanoparticles (TMV VNPs) were previously shown to promote osteogenic differentiation in vitro. This study aims to investigate whether and how TMV VNPs impact on osteoclastogenesis in vitro and bone injury healing in vivo. Methods: Raw264.7 cells were cultured in osteoclastogenic medium in culture plates coated with or without TMV and TMV-RGD1 VNPs, followed by TRAP staining, RT-qPCR and WB assessing expression of osteoclastogenic marker genes, and immunofluorescence assessing NF-kappa B activation. TMV and TMV-RGD1-modified hyaluronic acid hydrogel were used to treat mouse tibial bone injury. Bone injury healing was checked by micro-CT and Masson staining. Results: TMV and TMV-RGD1 VNPs significantly inhibited osteoclast differentiation and downregulated the expression of osteoclastogenic marker genes Ctr, Ctsk, Mmp-9, Rank, and Trap. Moreover, TMV and TMV-RGD1 VNPs inhibited NF-kappa B p65 phosphorylation and nuclear translocation, as well as activation of mTOR/AKT signaling pathway. TMV and TMV-RGD1-modified HA hydrogel strongly promoted mouse tibial bone injury with increased bone mass compared to plain HA hydrogel. The amount of osteoclasts was significantly reduced in TMV and TMV-RGD1 treated mice. TMV-RGD1 was more effective than TMV in inhibiting osteoclast differentiation and promoting bone injury repair. Discussion: These data demonstrated the great potential of TMV VNPs to be developed into biomaterial for bone injury repair or replacement.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60451]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Shan, Zhongshu,Bi, Hongtao,Suonan, Angxiu,et al. Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis Through Inhibiting mTOR/AKT Signaling[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2020,15.
APA	Shan, Zhongshu.,Bi, Hongtao.,Suonan, Angxiu.,Gu, Yong.,Zhou, Huan.,...&Chen, Liang.(2020).Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis Through Inhibiting mTOR/AKT Signaling.INTERNATIONAL JOURNAL OF NANOMEDICINE,15.
MLA	Shan, Zhongshu,et al."Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis Through Inhibiting mTOR/AKT Signaling".INTERNATIONAL JOURNAL OF NANOMEDICINE 15(2020).