Characterization and development of SSR markers of schizothoracine fish (Cypriniformes: Cyprinidae) based on SLAF-seq Technique

	Characterization and development of SSR markers of schizothoracine fish (Cypriniformes: Cyprinidae) based on SLAF-seq Technique
	Li, Xiaohui; Tang, Yongtao; Zhang, Renyi; Tian, Fei; Zhao, Kai
刊名	JOURNAL OF APPLIED ICHTHYOLOGY
	2020
卷号	36 期号:4
关键词	genetic diversity schizothoracine fish SLAF-seq Technique SSRs
英文摘要	Highly specialized grade of Schizothoracine fishe is primarily distributed in the drainage of Qinghai-Tibet Plateau and its surrounding region, which has precious biological resources. However, overfishing and habitat destruction have seriously affected the germplasm resources and population genetic structure of HSG, so it is a significance to search for reliable genetic markers to protect its wild resources. In this work, 27 novel polymorphic microsatellite were firstly generated for the entire HSG based on the SLAF data of the grade and assess their polymorphism in HSG from 4 genera of 8 species. Among which W24, W41, W50 were existed only in 3-4 populations with low amplification efficiency, and loci of W3, W6, W12, W25, W28, and W32 were absent in 1-3 species, the rest of that 18 loci were successfully amplified in all specimens. The average of the number of alleles (NA) per locus was 46.04, ranging from 12 to 79, and the average observed and expected heterozygosities were estimated from 0.539 to 0.785 and 0.471 to 0.669 in 8 species, respectively. The average polymorphic information content (PIC) of each species was from 0.669 to 0.433. The range of average Shannon-Weiner diversity index (H') were calculated from 0.914 to 1.717 in 8 species. To sum up, we developed 27 microsatellite loci with higher quality and stability, which could provide effective technical support for the study of germplasm resources of highly specialized grade of schizothoracine fishe.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60350]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Xiaohui,Tang, Yongtao,Zhang, Renyi,et al. Characterization and development of SSR markers of schizothoracine fish (Cypriniformes: Cyprinidae) based on SLAF-seq Technique[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2020,36(4).
APA	Li, Xiaohui,Tang, Yongtao,Zhang, Renyi,Tian, Fei,&Zhao, Kai.(2020).Characterization and development of SSR markers of schizothoracine fish (Cypriniformes: Cyprinidae) based on SLAF-seq Technique.JOURNAL OF APPLIED ICHTHYOLOGY,36(4).
MLA	Li, Xiaohui,et al."Characterization and development of SSR markers of schizothoracine fish (Cypriniformes: Cyprinidae) based on SLAF-seq Technique".JOURNAL OF APPLIED ICHTHYOLOGY 36.4(2020).