Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion

doi:10.3389/fmolb.2021.655662

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	Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion
	Li L(李龙)1,4; Wang XH(王小环)1,3,4; Wu, Helong 2; Shao YF(邵颖峰)1,4; Wu, Huaping 2; Song F(宋凡)1,3,4
刊名	FRONTIERS IN MOLECULAR BIOSCIENCES
	2021-04-12
卷号	8 页码:9
关键词	cell adhesion receptor-ligand binding nanoscale lipid cluster ligand mobility phase separation binding constant
DOI	10.3389/fmolb.2021.655662
通讯作者	Shao, Yingfeng(shaoyf@lnm.imech.ac.cn) ; Wu, Huaping(wuhuaping@gmail.com) ; Song, Fan(songf@lnm.imech.ac.cn)
英文摘要	Cell-cell adhesion and the adhesion of cells to extracellular matrix are mediated by the specific binding of receptors on the cell membrane to their cognate ligands on the opposing surface. The adhesion receptors can exhibit affinity for nanoscale lipid clusters that form in the cell membrane. Experimental studies of such adhesion systems often involve a cell adhering either to a solid surface with immobile ligands or a supported lipid bilayer with mobile ligands. A central question in these cell-substrate adhesions is how the mobility of the ligands physically affects their binding to the adhesion receptors and thereby the behavior of the nanoscale lipid clusters associated with the receptors. Using a statistical mechanical model and Monte Carlo simulations for the adhesion of cells to substrates with ligands, we find that, for mobile ligands, binding to adhesion receptors can promote the formation of mesoscale lipid domains, which in turn enhances the receptor-ligand binding. However, in the case of immobile ligands, the receptor-ligand binding and the tendency for the nanoscale lipid clusters to further coalesce depend on the distribution of the ligands on the substrate. Our findings help to explain why different adhesion experiments for identifying the interplay between receptor-ligand binding and heterogeneities in cell membranes led to contradictory results.
分类号	二类
资助项目	NSFC Basic Science Center Program for Multiscale Problems in Nonlinear Mechanics[11988102] ; Youth Innovation Promotion Association CAS ; National Natural Science Foundation of China[11902327] ; National Natural Science Foundation of China[11972041] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB22040102]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000643696100001
资助机构	NSFC Basic Science Center Program for Multiscale Problems in Nonlinear Mechanics ; Youth Innovation Promotion Association CAS ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences
其他责任者	Shao, Yingfeng ; Wu, Huaping ; Song, Fan
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/86500]
专题	力学研究所_非线性力学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Nonlinear Mech LNM, Inst Mech, Beijing, Peoples R China; 2.Zhejiang Univ Technol, Coll Mech Engn, Hangzhou, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing, Peoples R China;
推荐引用方式 GB/T 7714	Li L,Wang XH,Wu, Helong,et al. Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion[J]. FRONTIERS IN MOLECULAR BIOSCIENCES,2021,8:9.
APA	李龙,王小环,Wu, Helong,邵颖峰,Wu, Huaping,&宋凡.(2021).Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion.FRONTIERS IN MOLECULAR BIOSCIENCES,8,9.
MLA	李龙,et al."Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion".FRONTIERS IN MOLECULAR BIOSCIENCES 8(2021):9.