Flow field analyses of a porous membrane-separated, double-layered microfluidic chip for cell co-culture

doi:10.1007/s10409-020-00953-4

	Flow field analyses of a porous membrane-separated, double-layered microfluidic chip for cell co-culture
	Chen SB(陈深宝)3,4,6; Xue J(薛健)2,6; Hu JR(胡锦荣)3,4,6; Ding QH(丁奇寒)3,4,6; Zhou LW(周吕文)1,3,4; Feng SL(冯世亮)1,3,4; Cui YH 5; Lv SQ(吕守芹)3,4,6; Long M(龙勉)3,4,6
刊名	ACTA MECHANICA SINICA
	2020-05-09
页码	14
关键词	Immersed boundary method Porous membrane Wall shear stress Penetration velocity Microfluidic chip
ISSN号	0567-7718
DOI	10.1007/s10409-020-00953-4
英文摘要	Organs-on-chips composed of a porous membrane-separated, double-layered channels are used widely in elucidating the effects of cell co-culture and flow shear on biological functions. While the diversity of channel geometry and membrane permeability is applied, their quantitative correlation with flow features is still unclear. Immersed boundary methods (IBM) simulations and theoretical modelling were performed in this study. Numerical simulations showed that channel length, height and membrane permeability jointly regulated the flow features of flux, penetration velocity and wall shear stress (WSS). Increase of channel length, lower channel height or membrane permeability monotonically reduced the flow flux, velocity and WSS in upper channel before reaching a plateau. While the flow flux in lower channel monotonically increased with the increase of each factor, the WSS surprisingly exhibited a biphasic pattern with first increase and then decrease with increase of lower channel height. Moreover, the transition threshold of maximum WSS was sensitive to the channel length and membrane permeability. Theoretical modeling, integrating the transmembrane pressure difference and inlet flow flux with chip geometry and membrane permeability, was in good agreement with IBM simulations. These analyses provided theoretical bases for optimizing flow-specified chip design and evaluating flow microenvironments of in vivo tissue. Graphic
分类号	二类
WOS关键词	LATTICE BOLTZMANN METHOD ; RAT HEPATOCYTES ; MAINTENANCE ; SIMULATION ; MODEL
WOS研究方向	Engineering ; Mechanics
语种	英语
WOS记录号	WOS:000531215600001
资助机构	National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101]
其他责任者	Lu, Shouqin ; Long, Mian
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/84782]
专题	力学研究所_国家微重力实验室力学研究所_非线性力学国家重点实验室
作者单位	1.Ningbo Univ, Fac Mech Engn & Mech, Ningbo 315211, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Ctr Excellence Complex Syst Mech, Inst Mech, Beijing 100190, Peoples R China; 4.Chinese Acad Sci, Ctr Biomechan & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing Key Lab Engn Construct & Mech, Beijing 100190, Peoples R China; 5.Tianjin Univ, Dept Mech, Tianjin 300072, Peoples R China 6.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Chen SB,Xue J,Hu JR,et al. Flow field analyses of a porous membrane-separated, double-layered microfluidic chip for cell co-culture[J]. ACTA MECHANICA SINICA,2020:14.
APA	陈深宝.,薛健.,胡锦荣.,丁奇寒.,周吕文.,...&龙勉.(2020).Flow field analyses of a porous membrane-separated, double-layered microfluidic chip for cell co-culture.ACTA MECHANICA SINICA,14.
MLA	陈深宝,et al."Flow field analyses of a porous membrane-separated, double-layered microfluidic chip for cell co-culture".ACTA MECHANICA SINICA (2020):14.