Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?

doi:10.1021/acs.iecr.9b02094

	Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?
	Zhou, Fangfang 1,2; Luo, Jianquan 1,2; Qi, Benkun 1; Chen, Xiangrong 1; Wan, Yinhua 1,2
刊名	INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
	2019-07-10
卷号	58 期号:27 页码:11710-11719
ISSN号	0888-5885
DOI	10.1021/acs.iecr.9b02094
英文摘要	As the most dangerous carcinogens, efficient and green removal of Aflatoxin B1 (AFB1) is imperative. For the first time, horseradish peroxidase (HRP) was immobilized in/on an alginate/chitosan/montmorillonite (SA/CS/MON) hybrid microsphere and applied for the AFB1 removal. Compared to the enzyme immobilization via encapsulation and adsorption followed by cross-linking, HRP immobilized on the microsphere surface via covalent bonding showed the highest specific activity and AFB1 removal efficiency. The negatively charged MON could not only adsorb abundant AFB1 but also attract more CS chains on the microsphere surface and bring more active sites for enzyme immobilization and cross-linking; thus, the SA/CS/MON microspheres had stronger antiswelling ability and higher AFB1 adsorption capacity and enzyme loading. Although the AFB1 enrichment in the hybrid microsphere did not promote its degradation by enzymatic catalysis, the HRP-loaded hybrid microsphere could be reused and the enzymatic degradation of AFB1 increased a little as the adsorption became saturated. The enzymatic reaction was not enhanced by the substrate adsorption in this case because the AFB1 adsorption by the hybrid microspheres would decrease the AFB1 concentration in bulk solution and the degradation products might be adsorbed. The adsorbent (i.e., matrix for enzyme immobilization) has different adsorption ability to the substrate/product, and a catalyst with high activity on the substrate is desirable to maximize the synergistic effect of adsorption and catalysis. The obtained hybrid microsphere prototype is promising as a multifunctional matrix for many purposes since both the adsorbent and the catalyst can be designed.
资助项目	National Key Research and Development Plan of China[2017YFC1600906] ; Beijing Natural Science Foundation[2192053]
WOS关键词	CONTROLLED-RELEASE ; CALCIUM ALGINATE ; B-1 ; DEGRADATION ; DETOXIFICATION ; BEADS ; MEMBRANE ; RICE
WOS研究方向	Engineering
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000475537000010
资助机构	National Key Research and Development Plan of China ; Beijing Natural Science Foundation
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/30453]
专题	中国科学院过程工程研究所
通讯作者	Luo, Jianquan
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Fangfang,Luo, Jianquan,Qi, Benkun,et al. Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2019,58(27):11710-11719.
APA	Zhou, Fangfang,Luo, Jianquan,Qi, Benkun,Chen, Xiangrong,&Wan, Yinhua.(2019).Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,58(27),11710-11719.
MLA	Zhou, Fangfang,et al."Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 58.27(2019):11710-11719.