Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies

doi:10.1002/mbo3.581

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	Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies
	Zhong, Wenjing 1,2; Li, Guanghui 1; Yu, Xiaolu 1; Zhu, Min 1; Gong, Likun1 ; Wan, Yakun 1
刊名	MICROBIOLOGYOPEN
	2018-08
卷号	7 期号:4
关键词	biotin-streptavidin system Cry1B nanobody phage display sandwich ELISA
ISSN号	2045-8827
DOI	10.1002/mbo3.581
文献子类	Article
英文摘要	Bt Cry1B toxin, a residue in insect-resistant transgenic plants, has been identified to be harmful to human health. Therefore, it is urgent to detect the Cry1B toxin level in each kind of transgenic plant. Nbs, with prominently unique physiochemical properties, are becoming more and more promising tools in the detection of target antigens. In this study, an immune phage display library that was of high quality was successfully constructed for the screening of Cry1B-specific Nbs with excellent specificity, affinity, and thermostable. Subsequently, a novel sandwich ELISA for Cry1B detection was established, which was based on the biotin-streptavidin system using these afore-mentioned Nbs. This established detection system presented a linear working range from 5 to 1000 ng ml(-1) and a low detection limit of 3.46 ng ml(-1). The recoveries from spiked samples were in the range of 82.51%-113.56% with a relative standard deviation (RSD) lower than 5.00%. Taken together, the proposed sandwich ELISA would be a potential method for the detection of Cry1B toxin in transgenic Bt plants specifically and sensitively.
资助项目	Chinese Academy of Sciences[XDA12020332] ; Chinese Academy of Sciences[CASIMM0120164005] ; National Natural Science Foundation of China[31471216] ; National Natural Science Foundation of China[81673344]
WOS关键词	PHAGE-DISPLAYED LIBRARY ; DIRECTED EVOLUTION ; NANOBODY ; CONSTRUCTION ; IMMUNOASSAY ; CHEMISTRY ; PROTEINS ; AFFINITY
WOS研究方向	Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000440928500004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279637]
专题	药物安全性评价中心
通讯作者	Zhu, Min; Gong, Likun; Wan, Yakun
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Wenjing,Li, Guanghui,Yu, Xiaolu,et al. Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies[J]. MICROBIOLOGYOPEN,2018,7(4).
APA	Zhong, Wenjing,Li, Guanghui,Yu, Xiaolu,Zhu, Min,Gong, Likun,&Wan, Yakun.(2018).Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies.MICROBIOLOGYOPEN,7(4).
MLA	Zhong, Wenjing,et al."Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies".MICROBIOLOGYOPEN 7.4(2018).