Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats

doi:10.1293/tox.2013-0020

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	Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats
	Wu, Xiongfei; Li, Chenggang; Xing, Guozhen; Qi, Xinming; Ren, Jin
刊名	JOURNAL OF TOXICOLOGIC PATHOLOGY
	2013
卷号	26 期号:4 页码:385-392
关键词	resveratrol hepatocarcinogenesis laser microdissection GST-P Cyp2e1
ISSN号	0914-9198
DOI	10.1293/tox.2013-0020
文献子类	Article
英文摘要	Cyp2e1 plays an important role in chemically induced hepatocarcinogenesis. Resveratrol (REV) is known to prevent diethylnitrosamine (DEN)-induced hepatocarcinogenesis, but its effects on this process induced by DEN and 2-acetylaminofluorene (2-AAF) and the role of Cyp2e1 remain unclear. In this study, glutathione S-transferase placental form (GST-P)-positive foci were used as a marker of hepatocarcinogenesis. REV or diallyl disulfide (DADS, an inhibitor of Cyp2e1) significantly reduced both the area and number of GST-P-positive foci induced by DEN and 2-AAF. Treatment with REV or DADS also markedly decreased the expression of Cyp2e1 in the rat liver. By immunohistochemical staining of serial liver sections, we found that the expression of Cyp2e1 in GSTP-positive foci showed three distinct patterns: decreased in GST-P foci, increased in GST-P foci when compared with surrounding liver tissue and mixed type, The number of GST-P foci with increased Cyp2e1 expression was greater than the number of GST-P foci with decreased Cyp2e1. Protein levels of GST-P and Cyp2e1 were also higher in foci compared with surrounding liver tissue. REV or DADS significantly reduced the expression of GST-P and Cyp2e1 in both foci and surrounding liver tissue. Taken together, these results suggested that REV has a significant inhibitory effect on chemically induced hepatocarcinogenesis, which may be attributed to downregulation of Cyp2e1.
资助项目	National Key Technologies RD Program[2012ZX09302-003] ; National Key Technologies RD Program[2012ZX09301-001-006]
WOS关键词	TRANS-RESVERATROL ; HEPATOCELLULAR-CARCINOMA ; MEDIATED CHEMOPREVENTION ; PRENEOPLASTIC LESIONS ; BETA-NAPHTHOFLAVONE ; ANTIOXIDANT ENZYMES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; CYP1A2 INDUCER ; LIVER-DISEASE
WOS研究方向	Pathology ; Toxicology
语种	英语
出版者	JAPANESE SOC TOXICOLOGIC PATHOLOGY
WOS记录号	WOS:000330012200007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277345]
专题	药物安全性评价中心
通讯作者	Qi, Xinming
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xiongfei,Li, Chenggang,Xing, Guozhen,et al. Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats[J]. JOURNAL OF TOXICOLOGIC PATHOLOGY,2013,26(4):385-392.
APA	Wu, Xiongfei,Li, Chenggang,Xing, Guozhen,Qi, Xinming,&Ren, Jin.(2013).Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats.JOURNAL OF TOXICOLOGIC PATHOLOGY,26(4),385-392.
MLA	Wu, Xiongfei,et al."Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats".JOURNAL OF TOXICOLOGIC PATHOLOGY 26.4(2013):385-392.