Opposite regulation of hepatic breast cancer resistance protein in type 1 and 2 diabetes mellitus

doi:10.1016/j.ejphar.2013.12.008

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	Opposite regulation of hepatic breast cancer resistance protein in type 1 and 2 diabetes mellitus
	He, Lei 1,2; Yang, Yuan 3; Guo, Cen 1,2; Yao, Dan 2; Liu, Hui-hui 2; Sheng, Jing-jing 2; Zhou, Wei-ping3 ; Ren, Jin2 ; Liu, Xiao-dong 1; Pan, Guo-yu2
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2014-02-05
卷号	724 页码:185-192
关键词	Diabetes Sandwich cultured rat hepatocytes Breast cancer resistant protein
ISSN号	0014-2999
DOI	10.1016/j.ejphar.2013.12.008
文献子类	Article
英文摘要	Previous studies on diabetes have reported controversial results with regard to transporters in liver. The present study aimed to explore changes in hepatic breast cancer resistance protein (BCRP) expression and functions, as well as the possible underlying mechanisms, in type 2 diabetic patients, type 1 (streptozotocin-induced), and type 2 (Goto Kakizaki) diabetic rats. Protein and mRNA levels of human (h) and rat (r) BCRP were investigated using Western blot and quantitative polymerase chain reaction analyses. Functions of liver rBCRP were evaluated using rosuvastatin. Sandwich cultured rat hepatocytes (SCRH) were cultured with D-glucose, insulin, or oleic acid for 72 h, and rBCRP mRNA was detected. The effect of oleic acid on rBCRP function in SCRH was also investigated using rosuvastatin. Results showed that liver rBCRP mRNA levels decreased to 20% in type 1 diabetic rats, whereas that in diabetic patients and GK rats significantly increased threefold and twentyfold, respectively. No changes were observed in h/rBCRP protein levels of type 2 diabetic patients and GK rats. The functions of rBCRP significantly declined in type 1 diabetic rats but showed no significant. changes in GK rats. The data from SCRH indicated that D-glucose decreased rBCRP mRNA level to 60%. Oleic acid increased rBCRP mRNA in SCRH by approximately eightfold but decreased rBCRP function to 50%. Therefore, h/rBCRP expression and Functions were oppositely regulated in type 1 and type 2 diabetes mellitus subjects. Alternations in D-glucose, insulin, and free fatty acid levels in plasma might contribute to the changes in h/rBCRP expression and functions. (C) 2013 Elsevier B.V. All rights reserved
资助项目	National Science Foundation of China[81302836] ; Major National Science and Technology Programs[2012ZX09301001-006] ; Major National Science and Technology Programs[2012ZX09302003] ; National High Technology Research and Development Program of China[2013AA032202]
WOS关键词	DRUG-DRUG INTERACTIONS ; P-GLYCOPROTEIN ; BILIARY-EXCRETION ; RAT HEPATOCYTES ; IN-VIVO ; STREPTOZOTOCIN ; TRANSPORTER ; EXPRESSION ; CELLS ; MICE
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000331140500023
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277190]
专题	药物安全性评价中心
通讯作者	Liu, Xiao-dong
作者单位	1.China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China; 3.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai 200438, Peoples R China
推荐引用方式 GB/T 7714	He, Lei,Yang, Yuan,Guo, Cen,et al. Opposite regulation of hepatic breast cancer resistance protein in type 1 and 2 diabetes mellitus[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2014,724:185-192.
APA	He, Lei.,Yang, Yuan.,Guo, Cen.,Yao, Dan.,Liu, Hui-hui.,...&Pan, Guo-yu.(2014).Opposite regulation of hepatic breast cancer resistance protein in type 1 and 2 diabetes mellitus.EUROPEAN JOURNAL OF PHARMACOLOGY,724,185-192.
MLA	He, Lei,et al."Opposite regulation of hepatic breast cancer resistance protein in type 1 and 2 diabetes mellitus".EUROPEAN JOURNAL OF PHARMACOLOGY 724(2014):185-192.