Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study

doi:10.1109/TMECH.2013.2272562

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	Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study
	Zhang, Changlin 2,3; Li, Peng 2; Liu, Lianqing 2; Wang, Yuechao 2; Gao, Zhaobing1 ; Li, Guangyong 4
刊名	IEEE-ASME TRANSACTIONS ON MECHATRONICS
	2014-08
卷号	19 期号:4 页码:1138-1147
关键词	Atomic force microscope (AFM) mechanosensitive (MS) ion channel planar patch clamp
ISSN号	1083-4435
DOI	10.1109/TMECH.2013.2272562
文献子类	Article
英文摘要	Mechanosensitive ion channels play important roles for sensing and responding to the mechanical stimuli signals in living life. Here we report the development of a mechanostimulated patch-clamp system for simultaneous recording of external stimuli and acquisition of cellular physiological responses. This system integrates a custom-designed planar patch-clamp system with a robot-assisted atomic force microscope (AFM) system. The former, with a microfluidic channel, can realize not only recording electrical signals but also exchanging intracellular solution; while the latter, enhanced by robotic techniques (local scan force feedback, augmented reality vision feedback), can generate force stimuli with controllable patterns and magnitudes under the operator's real-time monitoring. To verify the performance of the developed system, we first measured the whole-cell current of the voltage-gated potassium ion channel Kv1.1 expressed on Human Embryonic Kidney (HEK293) cells and then recorded the mechanosensitive ion channel current in a mouse neuroblastoma cell line (Neuro2 A) in the whole-cell configuration during the AFM indenting on the membrane surface; finally, confirmed the ability to exchange intracellular solution by delivering propidium iodide into the captured cell through intracellular solution. The results prove the effectiveness of the system.
资助项目	National Natural Science Foundation of China[61175803] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201245] ; CAS/SAFEA International Partnership Program for Creative Research Teams[00000000] ; National Science Foundation[CNS 1035563]
WOS关键词	ATOMIC-FORCE MICROSCOPY ; GATED K+ CHANNELS ; MEMBRANE PATCHES ; SENSORY NEURONS ; SILICON CHIP ; ION CHANNELS ; LIVING CELLS ; WHOLE-CELL ; MECHANOTRANSDUCTION ; NANOMANIPULATION
WOS研究方向	Automation & Control Systems ; Engineering
语种	英语
出版者	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
WOS记录号	WOS:000335915800004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276976]
专题	神经药理学研究国际科学家工作站
通讯作者	Zhang, Changlin
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, State Key Lab Robot, Shenyang Inst Automat, Shenyang 110016, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Univ Pittsburgh, Dept Elect & Comp Engn, Pittsburgh, PA 15213 USA
推荐引用方式 GB/T 7714	Zhang, Changlin,Li, Peng,Liu, Lianqing,et al. Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study[J]. IEEE-ASME TRANSACTIONS ON MECHATRONICS,2014,19(4):1138-1147.
APA	Zhang, Changlin,Li, Peng,Liu, Lianqing,Wang, Yuechao,Gao, Zhaobing,&Li, Guangyong.(2014).Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study.IEEE-ASME TRANSACTIONS ON MECHATRONICS,19(4),1138-1147.
MLA	Zhang, Changlin,et al."Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study".IEEE-ASME TRANSACTIONS ON MECHATRONICS 19.4(2014):1138-1147.