Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3

doi:10.1038/s41401-018-0004-z

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	Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3
	Fang, Tian 3; Liu, Dan-Dan 1; Ning, He-Ming 1; Sun, Jing-Ya 1; Huang, Xiao-Jing 2; Dong, Yu 1; Geng, Mei-Yu1 ; Yun, Shi-Feng 3; Yan, Jun 2; Huang, Rui-Min1
刊名	Acta pharmacologica Sinica
	2018-12
卷号	39 期号:12 页码:1885-1893
ISSN号	1745-7254
DOI	10.1038/s41401-018-0004-z
文献子类	Article
英文摘要	Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P < 0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P < 0.05) and the increase of apoptosis index (P < 0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA database, we revealed that the overexpression of galectin-3 was associated with high tumor grade with lymph node metastasis, poor overall survival in UBC patients. Considering the remarkable inhibitory effects of MCP on UBC cell proliferation and survival in vitro and in vivo mainly through galectin-3, which is upregulated in UBCs, MCP may become an attractive agent, as a natural dietary fiber, for prevention and therapy of UBCs.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266767]
专题	分子影像中心（筹）
通讯作者	Yun, Shi-Feng; Yan, Jun; Huang, Rui-Min
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; 2.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, 210061, China 3.Department of Comparative Medicine, Jinling Hospital, Clinical School of Medical College of Nanjing University, Nanjing, 210002, China;
推荐引用方式 GB/T 7714	Fang, Tian,Liu, Dan-Dan,Ning, He-Ming,et al. Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3[J]. Acta pharmacologica Sinica,2018,39(12):1885-1893.
APA	Fang, Tian.,Liu, Dan-Dan.,Ning, He-Ming.,Sun, Jing-Ya.,Huang, Xiao-Jing.,...&Huang, Rui-Min.(2018).Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3.Acta pharmacologica Sinica,39(12),1885-1893.
MLA	Fang, Tian,et al."Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3".Acta pharmacologica Sinica 39.12(2018):1885-1893.