A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

doi:10.1002/bmc.4124

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	A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins
	Guo, Xiaozhen 1,3; Xie, Yang 1,2; Lian, Shan 1,2; Li, Zhixiong1,3 ; Gao, Yu 1,3; Xu, Zhou 1,3; Hu, Pei 1,3; Chen, Mingcang 1,3; Sun, Zhaolin 1,3; Tian, Xiaoting 1,3
刊名	BIOMEDICAL CHROMATOGRAPHY
	2018-03
卷号	32 期号:3
关键词	23-hydroxybetulinic acid HPLC-MS MS liver distribution pharmacokinetics Pulsatilla chinensis saponins
ISSN号	0269-3879
DOI	10.1002/bmc.4124
文献子类	Article
英文摘要	Pulsatilla chinensis saponins, the major active components in the herb, have drawn great attention as potential hepatitis B virus infection and hepatoma treatments. Here, a sensitive and accurate HPLC-MS/MS method was established for simultaneous determination of three saponins - anemoside B4, anemoside A3 and 23-hydroxybetulinic acid - in rat plasma and liver, and fully validated. The method was successfully applied to a pharmacokinetics and liver distribution study of P. chinensis saponins. Consequently, 23-hydroxybetulinic acid, with an extremely low content in the P. chinensis saponins, exhibited the highest exposure in the liver and in sites before and after hepatic disposition, namely, in the portal vein plasma and systemic plasma, followed by anemoside B4, which showed the highest content in the herb, whereas anemoside A3 displayed quite limited exposure. The hepatic first-pass effects were 71% for 23-hydroxybetulinic acid, 27% for anemoside B4 and 37% for anemoside A3, corresponding to their different extents of liver distribution. To our knowledge, this is the first investigation on the liver first-pass effect and distribution of P. chinensis saponins to date. These results also provide valuable information for the understanding of the pharmacological effect of P. chinensis saponins on liver diseases.
资助项目	Shanghai Science and Technology Foundation[14401900700] ; National Natural Science Foundation of China[81374052] ; National Natural Science Foundation of China for Young Scientists[81603280] ; National Natural Science Foundation of China for Young Scientists[81602998] ; China Postdoctoral Science Foundation[8160141707]
WOS关键词	MICROVASCULAR ENDOTHELIAL-CELLS ; INGREDIENTS INHIBIT SECRETION ; TRITERPENOIDAL SAPONINS ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; BUNGE REGEL ; HEPATITIS-B ; RAT ; APOPTOSIS ; ROOTS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY
WOS记录号	WOS:000424220400023
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279883]
专题	上海中药现代化研究中心
通讯作者	Tian, Xiaoting; Huang, Chenggang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Harbin Univ Commerce, Harbin, Heilongjiang, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China;
推荐引用方式 GB/T 7714	Guo, Xiaozhen,Xie, Yang,Lian, Shan,et al. A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins[J]. BIOMEDICAL CHROMATOGRAPHY,2018,32(3).
APA	Guo, Xiaozhen.,Xie, Yang.,Lian, Shan.,Li, Zhixiong.,Gao, Yu.,...&Huang, Chenggang.(2018).A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins.BIOMEDICAL CHROMATOGRAPHY,32(3).
MLA	Guo, Xiaozhen,et al."A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins".BIOMEDICAL CHROMATOGRAPHY 32.3(2018).