Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats

doi:10.1016/j.jchromb.2018.03.013

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	Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats
	Tian, Xiaoting 1,2; Xu, Zhou 1,2; Chen, Mingcang 1,2; Hu, Pei 1,2; Liu, Fang 1,2; Sun, Zhaolin 1,2; Liu, Huan 1,2; Guo, Xiaozheng 1,2; Li, Zhixiong1,2 ; Huang, Chenggang1,2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2018-05-01
卷号	1084 页码:122-131
关键词	Gouteng-Baitouweng LC-MS/MS Pharmacokinetics Liver Brain liver first-pass effect
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2018.03.013
文献子类	Article
英文摘要	Only focusing on the circulating levels is insufficient for the comprehensive understanding of the physiological disposition of herbal medicine in vivo. Therefore, we conducted the comprehensive investigation on the in vivo dynamic process of orally administrated Gouteng-Baitouweng (GB), a classical herb pair with anti-Parkinson potentials. Serving as the technical base, a sensitive and selective liquid chromatography-tandem mass spectrometry method was established and validated in the plasma, liver and brain, for simultaneous determination of five alkaloids (rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine and geissoschizine methyl ether) and three saponins (anemoside B4, anemoside A3 and 23-hydroxybetulinic acid). Following liquid-liquid extraction, favorable chromatographic behaviors of eight analytes were ob tamed on Waters Xbrigde C18 column within 13 min. This method elicited good linearity for the analytes at the concentration range of 0.3-1000 or 1.8-6000 ng/mL with favorable precision, accuracy and stability. Following oral administration of GB (25 g/kg) in rats, this method was applied to the quantitative analysis in the portal vein plasma, liver, systemic plasma, and brain. Consequently, anemoside B4 was of the highest exposure, followed by 23-hydroxybetulinic acid, anemoside A3, rhynchophylline and isocorynoxeine in vivo. Notably, three saponins were all observed with certain exposure in the brain, along with rhynchophylline at low levels. Besides, five alkaloids and 23-hydroxybetulinic acid underwent serious liver first-pass effect. Hence, the pharmacokinetics, liver first-pass effect, liver and brain distribution of ingredients in GB were clarified, which laid a solid foundation for interpreting its efficacy and safety.
资助项目	Shanghai Science and Technology Foundation[14401900700] ; National Natural Science Foundation of China[81374052] ; National Natural Science Foundation of China[81603280] ; National Natural Science Foundation of China[81602998] ; Strategic Biological Resources Service Network Plan of Chinese Academy of Sciences[ZSTH-015]
WOS关键词	LIQUID-CHROMATOGRAPHY ; PULSATILLA-CHINENSIS ; SYNAPTIC PLASTICITY ; MASS-SPECTROMETRY ; RHYNCHOPHYLLINE ; COMPONENTS ; CELLS ; METABOLITES ; DISEASE ; PLASMA
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000430524400015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279780]
专题	上海中药现代化研究中心
通讯作者	Li, Zhixiong; Huang, Chenggang
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Tian, Xiaoting,Xu, Zhou,Chen, Mingcang,et al. Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2018,1084:122-131.
APA	Tian, Xiaoting.,Xu, Zhou.,Chen, Mingcang.,Hu, Pei.,Liu, Fang.,...&Huang, Chenggang.(2018).Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1084,122-131.
MLA	Tian, Xiaoting,et al."Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1084(2018):122-131.