Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry

doi:10.1016/j.chroma.2008.08.097

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry
	Yang, Min 2; Sun, Jianghao 1; Lu, Zhiqiang 2; Chen, Guangtong 2; Guan, Shuhong2 ; Liu, Xuan 2; Jiang, Baohong2 ; Ye, Min 1; Guo, De-An1,2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2009-03-13
卷号	1216 期号:11 页码:2045-2062
关键词	Phytochemical analysis Traditional Chinese medicine HPLC-MS Tandem mass spectrometry Quality control
ISSN号	0021-9673
DOI	10.1016/j.chroma.2008.08.097
文献子类	Review
英文摘要	Traditional Chinese medicine (TCM) is commonly considered to operate due to the synergistic effects of all the major and minor components in the medicines. Hence sensitive and comprehensive analytical techniques are needed to acquire a better understanding of the pharmacological basis of the herb and to enhance the product quality control. The present review mainly focuses on the phytochemical analysis of TCMs using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) are the two commonly used ion sources. Triple quadrupole, ion trip (IT), Fourier transform ion cyclotron resonance (FTICR) and time-of-flight (TOF) mass spectrometers are used as on-line analyzer. The relationship between structural features and fragmentation patterns should be investigated as thoroughly as possible and hence be applied in the on-line analysis to deduce the Structures of detected peaks. Characteristic fragmentation behaviors of the reference standards, as well as information regarding polarity obtained from retention time data, on-line UV spectra, data from the literature and bio-sources of the compounds allowed the identification of the phytochemical constituents in the crude extracts. Although a mass spectrometer is not a universal detector, high-performance liquid chromatography Coupled with Multistage mass spectrometry (HPLC-MSn) technique was still proved to be a rapid and sensitive method to analyze the majority of the many constituents in herbal medicines, particularly for the detection of those present in minor or trace amounts. The methods established using HPLC-MS techniques facilitate the convenient and rapid quality control of traditional medicines and their pharmaceutical preparations. However, the quantitative analysis is not the topic of this review. (C) 2008 Elsevier B.V. All rights reserved.
资助项目	ministry of Sciences and Technology of China[2006BAI08B03-03]
WOS关键词	PRESSURE CHEMICAL-IONIZATION ; DIODE-ARRAY DETECTION ; OF-FLIGHT MASS ; RAPID STRUCTURAL-CHARACTERIZATION ; DIESTER-DITERPENOID ALKALOIDS ; RADIX SALVIA-MILTIORRHIZAE ; ZINGIBER-OFFICINALE ROSC. ; DAD-ESI-MSN ; ELECTROSPRAY-IONIZATION ; PHENOLIC-COMPOUNDS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000264226500010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279290]
专题	上海中药现代化研究中心
通讯作者	Guo, De-An
作者单位	1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Min,Sun, Jianghao,Lu, Zhiqiang,et al. Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2045-2062.
APA	Yang, Min.,Sun, Jianghao.,Lu, Zhiqiang.,Chen, Guangtong.,Guan, Shuhong.,...&Guo, De-An.(2009).Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1216(11),2045-2062.
MLA	Yang, Min,et al."Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1216.11(2009):2045-2062.