Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography
	Sha, Na 1,2; Huang, Hui-Lian 1,2; Zhang, Jin-Qiang 2; Chen, Guang-Tong 2; Tao, Si-Jia 2; Yang, Min 2; Li, Xing-Nuo 2; Li, Ping 1; Guo, De-An2
刊名	NATURAL PRODUCT COMMUNICATIONS
	2009-06
卷号	4 期号:6 页码:813-818
关键词	Propolis phenolic compounds R-P-HPLC quality control
ISSN号	1934-578X
文献子类	Article
英文摘要	A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed to simultaneously quantify the eight major bioactive phenolic compounds in Chinese propolis, namely caffeic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin 5-methyl ether, pinocembrin, benzyl caffeate, chrysin and galangin. This HPLC assay was performed on an Agilent Zorbax Extend-C-18 (250 x 4.6 mm, 5 mu m) column with a gradient of methanol and 0.2% aqueous acetic acid (v/v) in 50 min, at a flow rate of 1.0 mL/min, and detected at 290 rim. All calibration curves showed good linearity (r(2)>0.999) within the test ranges. The intra- and inter-day assay precision (RSD) of eight phenolic compounds were in the range of 0.07-4.92%. The recoveries were between 98.3% and 104.8%. This assay was applied to the evaluation of nineteen samples from different origins in China. The results indicated that the developed assay could be readily utilized for the quality control of propolis.
资助项目	Ministry of Science and Technology of China[2006BAI08B03-03] ; Shanghai Commission of Science and Technology[00000000]
WOS关键词	SALVIA-MILTIORRHIZA ; MEDICINAL PREPARATIONS ; BRAZILIAN PROPOLIS ; MASS-SPECTROMETRY ; PLANT-ORIGIN ; DAD METHOD ; HPLC-DAD ; CONSTITUENTS ; ANTIFUNGAL ; ROOTS
WOS研究方向	Pharmacology & Pharmacy ; Food Science & Technology
语种	英语
出版者	NATURAL PRODUCTS INC
WOS记录号	WOS:000267313900015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279231]
专题	上海中药现代化研究中心
通讯作者	Guo, De-An
作者单位	1.China Pharmaceut Univ, Coll Tradit Chinese Mat Med, Nanjing 210009, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Sha, Na,Huang, Hui-Lian,Zhang, Jin-Qiang,et al. Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography[J]. NATURAL PRODUCT COMMUNICATIONS,2009,4(6):813-818.
APA	Sha, Na.,Huang, Hui-Lian.,Zhang, Jin-Qiang.,Chen, Guang-Tong.,Tao, Si-Jia.,...&Guo, De-An.(2009).Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography.NATURAL PRODUCT COMMUNICATIONS,4(6),813-818.
MLA	Sha, Na,et al."Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography".NATURAL PRODUCT COMMUNICATIONS 4.6(2009):813-818.