Anti-Inflammatory Function of Withangulatin A by Targeted Inhibiting COX-2 Expression via MAPK and NF-kappa B Pathways

doi:10.1002/jcb.22430

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	Anti-Inflammatory Function of Withangulatin A by Targeted Inhibiting COX-2 Expression via MAPK and NF-kappa B Pathways
	Sun, Lijuan 2; Liu, Jianwen 2; Cui, Daling 2; Li, Jiyu 1; Yu, Youjun 2; Ma, Lei 2; Hu, Lihong 3
刊名	JOURNAL OF CELLULAR BIOCHEMISTRY
	2010-02-15
卷号	109 期号:3 页码:532-541
关键词	WITHANGULATIN A ANTI-INFLAMMATORY COX-2 MAPK NF-kappa B
ISSN号	0730-2312
DOI	10.1002/jcb.22430
文献子类	Article
英文摘要	Withangulatin A (WA), an active component isolated from Physalis angulata L., has been reported to possess anti-tumor and trypanocidal activities in model systems via multiple biochemical mechanisms. The aim of this study is to investigate its anti-inflammatory potential and the possible underlying mechanisms. In the current study, WA significantly suppressed mice T lymphocytes proliferation stimulated with LPS in a dose- and time-dependent manner and inhibited pro-inflammation cytokines (IL-2, IFN-gamma, and IL-6) dramatically. Moreover, WA targeted inhibited COX-2 expression mediated by MAPKs and NF-kappa B nuclear translocation pathways in mice T lymphocytes, and this result was further confirmed by the COX-1/2 luciferase reporter assay. Intriguingly, administration of WA inhibited the extent of mice ear swelling and decreased pro-inflammatory cytokines production in mice blood serum. Based on these evidences, WA influences the mice T lymphocytes function through targeted inhibiting COX-2 expression via MAPKs and NF-kappa B nuclear translocation signaling pathways, and this would make WA a strong candidate for further study as an anti-inflammatory agent. J. Cell. Biochem. 109: 532-541, 2010. (C) 2009 Wiley-Liss, Inc.
资助项目	China 111 Project[B07023] ; Science and Technology Commission of Shanghai Municipality[07DZ19716] ; Shanghai Leading Academic Discipline Project[B507]
WOS关键词	ACTIVATED PROTEIN-KINASE ; SMOOTH-MUSCLE-CELLS ; BRAIN TUMOR-CELLS ; PHYSALIS-ANGULATA ; CYCLOOXYGENASE-2 EXPRESSION ; RHEUMATOID-ARTHRITIS ; IMMUNE-RESPONSE ; TERMINAL KINASE ; NUCLEAR-FACTOR ; CANCER CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000274220000010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278971]
专题	上海中药现代化研究中心
通讯作者	Liu, Jianwen
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai 200092, Peoples R China; 2.E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Innovat Res Ctr Tradit Chinese Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lijuan,Liu, Jianwen,Cui, Daling,et al. Anti-Inflammatory Function of Withangulatin A by Targeted Inhibiting COX-2 Expression via MAPK and NF-kappa B Pathways[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2010,109(3):532-541.
APA	Sun, Lijuan.,Liu, Jianwen.,Cui, Daling.,Li, Jiyu.,Yu, Youjun.,...&Hu, Lihong.(2010).Anti-Inflammatory Function of Withangulatin A by Targeted Inhibiting COX-2 Expression via MAPK and NF-kappa B Pathways.JOURNAL OF CELLULAR BIOCHEMISTRY,109(3),532-541.
MLA	Sun, Lijuan,et al."Anti-Inflammatory Function of Withangulatin A by Targeted Inhibiting COX-2 Expression via MAPK and NF-kappa B Pathways".JOURNAL OF CELLULAR BIOCHEMISTRY 109.3(2010):532-541.