Metabolite Profiling and Characterization for Medicinal Herbal Remedies

doi:10.2174/1389200211209050535

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Metabolite Profiling and Characterization for Medicinal Herbal Remedies
	Yang, Min; Cheng, Chunru; Yang, Junling; Guo, De-an
刊名	CURRENT DRUG METABOLISM
	2012-06
卷号	13 期号:5 页码:535-557
关键词	Medicinal herbal remedy metabolite hyphenated technique complex system tandem mass spectrometry nuclear magnetic resonance identification preparation
ISSN号	1389-2002
DOI	10.2174/1389200211209050535
文献子类	Review
英文摘要	Metabolisms of herbal remedies and their natural components, which play a critical role in support of medicine development, and clinical medication, are drastically different from those of designed drugs. The separation, isolation and identification of drug metabolites from complex endogenous matrices like urine, plasma and tissue extracts are extremely challenging. For herbal medicine studies, it is even more difficult due to the complex chemical composition. Usually, a combination of high performance liquid chromatography (HPLC) and mass spectrometry (MS) is proven to be a powerful analytical tool for screening and identifying drug metabolites. For suitable instruments, the quadrupole time-of-flight (Q-TOF), hybrid ion trap time-of-flight (IT-TOF), and orbitrap mass spectrometry could clearly enhance the efficiency in metabolite profiling compared to general triquadrupole (QQQ) and ion trap (IT) mass spectrometry technique. Due to the ability for unambiguous structure determination, nuclear magnetic resonance spectroscopy (NMR) is also coupled to HPLC for on-line analysis of metabolites. Capillary electrophoresis and gas chromatography are also optional methods. These techniques could provide abundant information from a wide variety of samples. However, in many cases, preparations of metabolites are critical for further pharmacokinetics, pharmacologic, and toxic evaluation of the remedy. Therefore, accumulations of metabolites from the in vivo biological samples are essential. Biotransformation models are considered to be important complementary sources for preparation of drug metabolites. Fungi, plant cells, and a variety of enzymes were used to provide information for further in vivo testing. This review focuses on the screening and identification of drug metabolites of herbal medicines.
资助项目	National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program'[2009ZX09304-002] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program'[2009ZX09308-005] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program'[2009ZX09311-001] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program'[2009ZX09102-122] ; Natural Science Foundation of Shanghai, China[10ZR1436400]
WOS关键词	TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; RAT-LIVER MICROSOMES ; TRADITIONAL CHINESE MEDICINES ; ION-CYCLOTRON RESONANCE ; DIODE-ARRAY DETECTION ; PHASE-II METABOLITES ; COLLISION-INDUCED DISSOCIATION ; ZINGIBER-OFFICINALE ROSC. ; IN-VITRO METABOLISM
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000304041700005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278069]
专题	上海中药现代化研究中心
通讯作者	Guo, De-an
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Min,Cheng, Chunru,Yang, Junling,et al. Metabolite Profiling and Characterization for Medicinal Herbal Remedies[J]. CURRENT DRUG METABOLISM,2012,13(5):535-557.
APA	Yang, Min,Cheng, Chunru,Yang, Junling,&Guo, De-an.(2012).Metabolite Profiling and Characterization for Medicinal Herbal Remedies.CURRENT DRUG METABOLISM,13(5),535-557.
MLA	Yang, Min,et al."Metabolite Profiling and Characterization for Medicinal Herbal Remedies".CURRENT DRUG METABOLISM 13.5(2012):535-557.